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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Protein C inhibitor Monoclonal antibody

Protein C inhibitor Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2H6G2

Cat no : 66030-1-Ig

Synonyms

PAI3, PCI, PLANH3, PROCI, Protein C inhibitor, SERPINA5

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using Protein C inhibitor Monoclonal antibody (66030-1-Ig)

    WB analysis of human testis using 66030-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using Protein C inhibitor Monoclonal antibody (66030-1-Ig)

    WB analysis of human testis using 66030-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using Protein C inhibitor Monoclonal antibody (66030-1-Ig)

    WB analysis of human spleen using 66030-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using Protein C inhibitor Monoclonal antibody (66030-1-Ig)

    WB analysis of human spleen using 66030-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Protein C inhibitor Monoclonal antibody (66030-1-Ig)

    IHC staining of human ovary tumor using 66030-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66030-1-Ig (Protein C inhibitor antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Protein C inhibitor Monoclonal antibody (66030-1-Ig)

    IHC staining of human ovary tumor using 66030-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66030-1-Ig (Protein C inhibitor antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using Protein C inhibitor Monoclonal antibody (66030-1-Ig)

    IF Staining of human ovary tumor using 66030-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using Protein C inhibitor antibody (66030-1-Ig, Clone: 2H6G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using Protein C inhibitor Monoclonal antibody (66030-1-Ig)

    IF Staining of human ovary tumor using 66030-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using Protein C inhibitor antibody (66030-1-Ig, Clone: 2H6G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHuman testis, tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman ovary tumor tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66030-1-Ig is Protein C inhibitor Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Protein C inhibitor fusion protein Ag18094 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC008915
Gene symbol SERPINA5
Gene ID (NCBI) 5104
RRIDAB_11045661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINA5 (also called Protein C Inhibitor, PCI ) is a serpin type of serine protease inhibitor that is found in many tissues and fluids in human, including blood plasma, seminal plasma and urine. PCI found in blood originates from the liver and is capable of inhibiting several serine proteases involved in the regulation of coagulation and fibrinolysis, including activated protein C, thrombin, factor Xa, various kallikreins and plasminogen activators. PCI has been found to have antimicrobial and antitumor properties and thus appears to be a multi-functional protein.

Protocols

Product Specific Protocols
WB protocol for Protein C inhibitor antibody 66030-1-IgDownload protocol
IHC protocol for Protein C inhibitor antibody 66030-1-IgDownload protocol
IF protocol for Protein C inhibitor antibody 66030-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols