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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SERPINA7 Monoclonal antibody

SERPINA7 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1B11B10

Cat no : 66454-1-Ig

Synonyms

Serpin A7, SERPINA7, T4 binding globulin, TBG, Thyroxine binding globulin

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using SERPINA7 Monoclonal antibody (66454-1-Ig)

    WB analysis of human plasma using 66454-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66454-1-Ig (SERPINA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using SERPINA7 Monoclonal antibody (66454-1-Ig)

    WB analysis of human testis using 66454-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66454-1-Ig (SERPINA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SERPINA7 Monoclonal antibody (66454-1-Ig)

    IHC staining of human liver cancer using 66454-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SERPINA7 Monoclonal antibody (66454-1-Ig)

    IHC staining of human liver cancer using 66454-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SERPINA7 Monoclonal antibody (66454-1-Ig)

    IHC staining of mouse liver using 66454-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SERPINA7 Monoclonal antibody (66454-1-Ig)

    IHC staining of mouse liver using 66454-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using SERPINA7 Monoclonal antibody (66454-1-Ig)

    IF Staining of human liver cancer using 66454-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERPINA7 antibody (66454-1-Ig, Clone: 1B11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using SERPINA7 Monoclonal antibody (66454-1-Ig)

    IF Staining of human liver cancer using 66454-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERPINA7 antibody (66454-1-Ig, Clone: 1B11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman plasma tissue, human testis tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

66454-1-Ig targets SERPINA7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SERPINA7 fusion protein Ag9823 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
Calculated molecular weight 415 aa, 46 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC020747
Gene symbol SERPINA7
Gene ID (NCBI) 6906
RRIDAB_2881823
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SERPINA7 antibody 66454-1-IgDownload protocol
IHC protocol for SERPINA7 antibody 66454-1-IgDownload protocol
IF protocol for SERPINA7 antibody 66454-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Heliyon

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study

Authors - Lingyan Chen
View Article