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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Maspin Monoclonal antibody

Maspin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3G5A3

Cat no : 67633-1-Ig

Synonyms

maspin, Peptidase inhibitor 5, PI 5, PI5, Serpin B5, SERPINB5, SPB5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Maspin Monoclonal antibody (67633-1-Ig)

    WB analysis using 67633-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67633-1-Ig (Maspin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUMAN saliva using Maspin Monoclonal antibody (67633-1-Ig)

    WB analysis of HUMAN saliva using 67633-1-Ig

    Human saliva were subjected to SDS PAGE followed by western blot with 67633-1-Ig (Maspin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Maspin Monoclonal antibody (67633-1-Ig)

    IHC staining of human oesophagus cancer using 67633-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67633-1-Ig (Maspin antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Maspin Monoclonal antibody (67633-1-Ig)

    IHC staining of human oesophagus cancer using 67633-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67633-1-Ig (Maspin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Maspin Monoclonal antibody (67633-1-Ig)

    IF Staining of HEK-293 using 67633-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Maspin antibody (67633-1-Ig, Clone: 3G5A3 ) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inHepG2 cells, Human saliva, HeLa cells, A431 cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67633-1-Ig targets Maspin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Maspin fusion protein Ag19129 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 5
Calculated molecular weight 375 aa, 42 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC020713
Gene symbol Maspin
Gene ID (NCBI) 5268
RRIDAB_2882834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINB5, also named as PI-5, Maspin and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.

Protocols

Product Specific Protocols
WB protocol for Maspin antibody 67633-1-IgDownload protocol
IHC protocol for Maspin antibody 67633-1-IgDownload protocol
IF protocol for Maspin antibody 67633-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols