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  • Featured Product
  • KD/KO Validated

SERPINB8 Polyclonal antibody

SERPINB8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13102-2-AP

Synonyms

PI8, Serpin B8, PI 8, Peptidase inhibitor 8, Cytoplasmic antiproteinase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SERPINB8 Polyclonal antibody (13102-2-AP)

    WB analysis of Jurkat using 13102-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13102-2-AP (SERPINB8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SERPINB8 Polyclonal antibody (13102-2-AP)

    WB analysis using 13102-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13102-2-AP (SERPINB8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using SERPINB8 Polyclonal antibody (13102-2-AP)

    WB analysis of mouse lung using 13102-2-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13102-2-AP (SERPINB8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using SERPINB8 Polyclonal antibody (13102-2-AP)

    IHC staining of human oesophagus cancer using 13102-2-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13102-2-AP (SERPINB8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using SERPINB8 Polyclonal antibody (13102-2-AP)

    IHC staining of human oesophagus cancer using 13102-2-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13102-2-AP (SERPINB8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, mouse lung tissue
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13102-2-AP targets SERPINB8 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERPINB8 fusion protein Ag3740 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 8
Calculated molecular weight 374 aa, 43 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC034528
Gene symbol SERPINB8
Gene ID (NCBI) 5271
RRIDAB_2186472
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SERPINB8 antibody 13102-2-APDownload protocol
IHC protocol for SERPINB8 antibody 13102-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Dermatol

SERPINB8 and furin regulate ITGAX expression and affect the proliferation and invasion of melanoma cells

Authors - Li Ni
  • KD Validated
View Article