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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SERPIND1 Monoclonal antibody

SERPIND1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C1E1

Cat no : 67602-1-Ig

Synonyms

D22S673, HC II, HC2, HCF2, HCII, Heparin cofactor 2, Heparin cofactor II, HLS2, LS2, Protease inhibitor leuserpin 2, Serpin D1, SERPIND1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SERPIND1 Monoclonal antibody (67602-1-Ig)

    WB analysis using 67602-1-Ig

    0.8 μL, 0.4 μL, and 0.2 μL human plasma lysates were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig plasma using SERPIND1 Monoclonal antibody (67602-1-Ig)

    WB analysis of pig plasma using 67602-1-Ig

    0.4 μL pig plasma were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using SERPIND1 Monoclonal antibody (67602-1-Ig)

    WB analysis of human plasma using 67602-1-Ig

    0.2 μLhuman plasma were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using SERPIND1 Monoclonal antibody (67602-1-Ig)

    WB analysis of human plasma using 67602-1-Ig

    0.4 μL human plasma were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SERPIND1 Monoclonal antibody (67602-1-Ig)

    WB analysis of human placenta using 67602-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using SERPIND1 Monoclonal antibody (67602-1-Ig)

    IHC staining of human liver using 67602-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67602-1-Ig (SERPIND1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SERPIND1 Monoclonal antibody (67602-1-Ig)

    IHC staining of human liver using 67602-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67602-1-Ig (SERPIND1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SERPIND1 Monoclonal antibody (67602-1-Ig)

    IF Staining of HepG2 using 67602-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SERPIND1 antibody (67602-1-Ig, Clone: 2C1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman plasma, human placenta tissue, pig plasma
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67602-1-Ig targets SERPIND1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SERPIND1 fusion protein Ag26351 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade D (heparin cofactor), member 1
Calculated molecular weight 499 aa, 57 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC035028
Gene symbol SERPIND1
Gene ID (NCBI) 3053
RRIDAB_2882808
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SERPIND1 antibody 67602-1-IgDownload protocol
IHC protocol for SERPIND1 antibody 67602-1-IgDownload protocol
IF protocol for SERPIND1 antibody 67602-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols