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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SERPINE2 Monoclonal antibody, PBS Only

SERPINE2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11F12

Cat no : 66203-1-PBS

Synonyms

GDN, Glia derived nexin, Peptidase inhibitor 7, PI 7, PI7, PN 1, PN1, PN-1, PNI, Protease nexin 1, Protease nexin I, Serpin E2, SERPINE2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SERPINE2 Monoclonal antibody (66203-1-Ig)

    WB analysis using 66203-1-Ig (same clone as 66203-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SERPINE2 Monoclonal antibody (66203-1-Ig)

    WB analysis of HEK-293 using 66203-1-Ig (same clone as 66203-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using SERPINE2 Monoclonal antibody (66203-1-Ig)

    WB analysis of Neuro-2a using 66203-1-Ig (same clone as 66203-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using SERPINE2 Monoclonal antibody (66203-1-Ig)

    WB analysis of C6 using 66203-1-Ig (same clone as 66203-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SERPINE2 Monoclonal antibody (66203-1-Ig)

    IHC staining of human placenta using 66203-1-Ig (same clone as 66203-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SERPINE2 Monoclonal antibody (66203-1-Ig)

    IHC staining of human placenta using 66203-1-Ig (same clone as 66203-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SERPINE2 Monoclonal antibody (66203-1-Ig)

    IHC staining of human testis using 66203-1-Ig (same clone as 66203-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SERPINE2 Monoclonal antibody (66203-1-Ig)

    IHC staining of human testis using 66203-1-Ig (same clone as 66203-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SERPINE2 Monoclonal antibody (66203-1-Ig)

    IF Staining of HEK-293 using 66203-1-Ig (same clone as 66203-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SERPINE2 antibody (66203-1-Ig, Clone: 1E11F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66203-1-PBS targets SERPINE2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SERPINE2 fusion protein Ag1830 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC015663
Gene symbol SERPINE2
Gene ID (NCBI) 5270
RRIDAB_2881594
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.