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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SERPING1/C1 Inactivator Monoclonal antibody (66882-1-Ig)

    WB analysis using 66882-1-Ig (same clone as 66882-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66882-1-Ig (SERPING1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66882-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SERPING1/C1 Inactivator Monoclonal antibody (66882-1-Ig)

    IF Staining of HeLa using 66882-1-Ig (same clone as 66882-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66882-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SERPING1/C1 Inactivator Monoclonal antibody (66882-1-Ig)

    IF Staining of HepG2 using 66882-1-Ig (same clone as 66882-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66882-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SERPING1/C1 Inactivator Monoclonal antibody (66882-1-Ig)

    IF Staining of HepG2 using 66882-1-Ig (same clone as 66882-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66882-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

SERPING1/C1 Inactivator Monoclonal antibody, PBS Only

SERPING1/C1 Inactivator Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66882-1-PBS
Clone No.1B11B11

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

C1 esterase inhibitor, C1 Inh, C1 inhibiting factor, C1IN, C1INH, C1NH, HAE1, HAE2, Plasma protease C1 inhibitor, Serpin G1, SERPING1, SERPING1/C1 Inactivator

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66882-1-PBS targets SERPING1/C1 Inactivator in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig, rat samples.

Tested Reactivity Human, pig, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SERPING1/C1 Inactivator fusion protein Ag16782 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
Calculated molecular weight 500 aa, 55 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC011171
Gene Symbol SERPING1
Gene ID (NCBI) 710
RRIDAB_2882214
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05155
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SERPING1, also known as C1 Inhibitor (C1INH), is a member of the Serine proteinase inhibitor family. Its main function is the inhibition of the complement system to prevent spontaneous activation. It inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). C1INH is the most heavily glycosylated plasma protein.