Validation Data Gallery
Tested Applications
| Positive WB detected in | human skeletal muscle tissue, HEK-293 cells, mouse liver tissue, human testis tissue, HeLa cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 23 publications below |
| IHC | See 2 publications below |
Product Information
21668-1-AP targets SESN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16462 Product name: Recombinant human SESN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-141 aa of BC112036 Sequence: MAEGENEVRWDGLCSRDSTTRETALENIRQTILRKTEYLRSVKETPHRPSDGLSNTESSDGLNKLLAHLLMLSKRCPFKDVREKSEFILKSIQELGIRIPRPLGQGPSRFIPEKEILQVGSEDAQMHALFADSFAALGRLD 相同性解析による交差性が予測される生物種 |
| Full Name | sestrin 1 |
| Calculated molecular weight | 551 aa, 64 kDa |
| Observed molecular weight | 66-68 kDa |
| GenBank accession number | BC112036 |
| Gene Symbol | SESN1 |
| Gene ID (NCBI) | 27244 |
| RRID | AB_10793724 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6P5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 1 is implicated in the inhibition of cell growth. It is approximately a 66kDa protein in humans (551 amino acids).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SESN1 antibody 21668-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine. | ||
Proc Natl Acad Sci U S A Rag-Ragulator is the central organizer of the physical architecture of the mTORC1 nutrient-sensing pathway | ||
Biochim Biophys Acta Mol Basis Dis Exercise ameliorates chronic inflammatory response induced by high-fat diet via Sestrin2 in an Nrf2-dependent manner | ||
Osteoarthritis Cartilage Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism. |
