Featured Product
KD/KO Validated

Sestrin 2 Polyclonal antibody

Name: Sestrin 2 Polyclonal antibody
Brand: Proteintech
SKU: 21346-1-AP

Sestrin 2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21346-1-AP

Synonyms

SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene

Validation Data Gallery

Filter:

AllWBIF/ICC

WB analysis of mouse thymus using 21346-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 21346-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21346-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 21346-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 21346-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IF Staining of U2OS using 21346-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Sestrin 2 antibody (21346-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Cat No. 21346-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse thymus tissue, HeLa cells, K-562 cells, mouse lung tissue, NIH/3T3 cells
Positive IF/ICC detected in	U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 11 publications below
IF	See 5 publications below

Product Information

21346-1-AP targets Sestrin 2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Sestrin 2 fusion protein Ag15669 相同性解析による交差性が予測される生物種
Full Name	sestrin 2
Calculated molecular weight	480 aa, 54 kDa
Observed molecular weight	60-66 kDa
GenBank accession number	NM_031459
Gene symbol	SESN2/Sestrin 2
Gene ID (NCBI)	83667
RRID	AB_10733238
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

Protocols

Product Specific Protocols
WB protocol for Sestrin 2 antibody 21346-1-AP	Download protocol
IF protocol for Sestrin 2 antibody 21346-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors. Authors - Alessandro Carugo View Article
human	WB	Elife Dynamics of mTORC1 activation in response to amino acids. Authors - Maria Manifava KD Validated View Article
mouse	WB,IF	Front Immunol Sestrin2 provides cerebral protection through activation of Nrf2 signaling in microglia following subarachnoid hemorrhage Authors - Youqing Yang View Article
human	WB,IF	J Invest Dermatol Metastatic melanoma cells rely on Sestrin2 to acquire anoikis resistance via detoxifying intracellular ROS. Authors - Guannan Zhu KD Validated View Article
rat	WB	Mol Cell Biol Sestrin2 protects dopaminergic cells against rotenone toxicity through AMPK-dependent autophagy activation. Authors - Yi-Sheng Hou View Article
rat	IF	J Cereb Blood Flow Metab Sestrin2, as a negative feedback regulator of mTOR, provides neuroprotection by activation AMPK phosphorylation in neonatal hypoxic-ischemic encephalopathy in rat pups. Authors - Xudan Shi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Sestrin 2 Polyclonal antibody

Sestrin 2 Polyclonal Antibody for WB, IF/ICC, ELISA

Validation Data Gallery

WB analysis of mouse thymus using 21346-1-AP

WB analysis of K-562 using 21346-1-AP

WB analysis of HeLa using 21346-1-AP

WB analysis of NIH/3T3 using 21346-1-AP

WB analysis of mouse lung using 21346-1-AP

IF Staining of U2OS using 21346-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Dynamics of mTORC1 activation in response to amino acids.

Sestrin2 provides cerebral protection through activation of Nrf2 signaling in microglia following subarachnoid hemorrhage

Metastatic melanoma cells rely on Sestrin2 to acquire anoikis resistance via detoxifying intracellular ROS.

Sestrin2 protects dopaminergic cells against rotenone toxicity through AMPK-dependent autophagy activation.

Sestrin2, as a negative feedback regulator of mTOR, provides neuroprotection by activation AMPK phosphorylation in neonatal hypoxic-ischemic encephalopathy in rat pups.