Validation Data Gallery

Filter: AllWB

WB analysis of HeLa using 11431-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11431-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11431-2-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11431-2-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 11431-2-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11431-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11431-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 11431-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11431-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11431-2-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11431-2-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11431-2-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11431-2-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Featured Product
KD/KO Validated

SESN3 Polyclonal antibody

SESN3 Polyclonal Antibody for WB, ELISA

Cat No. 11431-2-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Sestrin-3, sestrin 3, SEST3, EC:1.11.1.-

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (17)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

HeLa cells, A431 cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse ovary tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 14 publications below
IHC	See 3 publications below

Product Information

11431-2-AP targets SESN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SESN3 fusion protein Ag1961 相同性解析による交差性が予測される生物種
Full Name	sestrin 3
Calculated molecular weight	492 aa, 57 kDa
Observed molecular weight	36 kDa, 51-59 kDa
GenBank accession number	BC017296
Gene Symbol	SESN3
Gene ID (NCBI)	143686
RRID	AB_2185602
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P58005
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity.

Protocols

Product Specific Protocols
WB protocol for SESN3 antibody 11431-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Dev Cell FoxOs inhibit mTORC1 and activate Akt by inducing the expression of Sestrin3 and Rictor. Authors - Chia-Chen Chen KD Validated View Article
mouse	IHC	Proc Natl Acad Sci U S A FOXO1 and FOXO3 transcription factors have unique functions in meniscus development and homeostasis during aging and osteoarthritis. Authors - Kwang Il Lee View Article
mouse	WB	Cell Death Dis Pifithrin-μ sensitizes mTOR-activated liver cancer to sorafenib treatment Authors - Jiarui Lv View Article
human	WB	Elife Selective eradication of cancer displaying hyperactive Akt by exploiting the metabolic consequences of Akt activation. Authors - Veronique Nogueira KD Validated View Article
mouse	WB	Biochim Biophys Acta Mol Basis Dis Exercise ameliorates chronic inflammatory response induced by high-fat diet via Sestrin2 in an Nrf2-dependent manner Authors - Sujuan Liu View Article
human	WB,IHC	Osteoarthritis Cartilage Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism. Authors - T Shen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 11431-2-AP

WB analysis of HeLa using 11431-2-AP

WB analysis of HEK-293 using 11431-2-AP

WB analysis of HEK-293 using 11431-2-AP

WB analysis of mouse ovary using 11431-2-AP

WB analysis of HepG2 using 11431-2-AP

WB analysis of HeLa using 11431-2-AP

WB analysis of A549 using 11431-2-AP

WB analysis of HeLa using 11431-2-AP

WB analysis of mouse liver using 11431-2-AP

WB analysis of mouse liver using 11431-2-AP

WB analysis of A431 using 11431-2-AP

WB analysis of A431 using 11431-2-AP

SESN3 Polyclonal antibody

SESN3 Polyclonal Antibody for WB, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

FoxOs inhibit mTORC1 and activate Akt by inducing the expression of Sestrin3 and Rictor.

FOXO1 and FOXO3 transcription factors have unique functions in meniscus development and homeostasis during aging and osteoarthritis.

Pifithrin-μ sensitizes mTOR-activated liver cancer to sorafenib treatment

Selective eradication of cancer displaying hyperactive Akt by exploiting the metabolic consequences of Akt activation.

Exercise ameliorates chronic inflammatory response induced by high-fat diet via Sestrin2 in an Nrf2-dependent manner

Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.