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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SOLO Polyclonal antibody

SOLO Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 23911-1-AP

Synonyms

SESTD1, SEC14 domain and spectrin repeat-containing protein 1, SEC14 and spectrin domains 1, Protein Solo, Huntingtin-interacting protein-like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOLO Polyclonal antibody (23911-1-AP)

    WB analysis using 23911-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23911-1-AP (SOLO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using SOLO Polyclonal antibody (23911-1-AP)

    WB analysis of rat brain using 23911-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 23911-1-AP (SOLO antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SOLO Polyclonal antibody (23911-1-AP)

    IP experiment of mouse brain using 23911-1-AP

    IP result of anti-SOLO (IP:23911-1-AP, 4ug; Detection:23911-1-AP 1:4000) with mouse brain tissue lysate 1280 ug.

  • Immunoprecipitation (IP) experiment of rat brain tissue using SOLO Polyclonal antibody (23911-1-AP)

    IP experiment of rat brain using 23911-1-AP

    IP result of anti-SOLO (IP:23911-1-AP, 0ug; Detection:23911-1-AP 1:4000) with rat brain tissue lysate 1120 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SOLO Polyclonal antibody (23911-1-AP)

    IF Staining of HepG2 using 23911-1-AP

    Immunofluorescent analysis of HepG2 cells using 23911-1-AP (SOLO antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23911-1-AP targets SOLO in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOLO fusion protein Ag21016 相同性解析による交差性が予測される生物種
Full Name SEC14 and spectrin domains 1
Calculated molecular weight 696 aa, 79 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC047578
Gene symbol SOLO
Gene ID (NCBI) 91404
RRIDAB_2879360
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SOLO antibody 23911-1-APDownload protocol
IF protocol for SOLO antibody 23911-1-APDownload protocol
IP protocol for SOLO antibody 23911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols