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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SET Monoclonal antibody (67478-1-Ig)

    WB analysis of Jurkat using 67478-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using SET Monoclonal antibody (67478-1-Ig)

    WB analysis of HSC-T6 using 67478-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using SET Monoclonal antibody (67478-1-Ig)

    WB analysis of 4T1 using 67478-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SET Monoclonal antibody (67478-1-Ig)

    WB analysis of HeLa using 67478-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SET Monoclonal antibody (67478-1-Ig)

    WB analysis of HepG2 using 67478-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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SET Monoclonal antibody

SET Monoclonal Antibody for WB, ELISA
Cat No. 67478-1-Ig
Clone No.2B4D1
Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Synonyms

2PP2A, HLA DR associated protein II, I 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, Protein SET, SET, SET nuclear oncogene, TAF I, TAF IBETA, Template activating factor I

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, 4T1 cells, HeLa cells, HepG2 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67478-1-Ig targets SET in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SET nuclear oncogene
Calculated molecular weight 33 kDa
Observed molecular weight 33-36 kDa
GenBank accession numberNM_003011
Gene Symbol SET
Gene ID (NCBI) 6418
RRIDAB_2882705
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01105
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

SET, also named as IGAAD, I-2PP2A, TAF-I, IGAAD and PHAPII, belongs to the nucleosome assembly protein (NAP) family.SET is a multifunctional oncoprotein which is ubiquitously expressed in all kinds of cells. The SET protein participates in many cellular processes including cell cycle, cell migration, apoptosis, transcription, and DNA repair. The SET protein plays important roles in facilitating cell proliferation via many pathways that promote tumorigenesis and metastasis.

Protocols

Product Specific Protocols
WB protocol for SET antibody 67478-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression

Authors - Ling Zhang
View Article