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  • Featured Product
  • KD/KO Validated

SETD1A Monoclonal antibody, PBS Only

SETD1A Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C2G12

Cat no : 67936-1-PBS

Synonyms

hSET1A, KIAA0339, KMT2F, Lysine N methyltransferase 2F, SET domain containing 1A, Set1, Set1A, SETD1A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SETD1A Monoclonal antibody (67936-1-Ig)

    WB analysis using 67936-1-Ig (same clone as 67936-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SETD1A Monoclonal antibody (67936-1-Ig)

    WB analysis of HEK-293 using 67936-1-Ig (same clone as 67936-1-PBS)

    WB result of SETD1A antibody (67936-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SETD1A transfected HEK-293 cells. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SETD1A Monoclonal antibody (67936-1-Ig)

    WB analysis of mouse brain using 67936-1-Ig (same clone as 67936-1-PBS)

    Mouse brain tissue was subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at a dilution of 1:3000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using SETD1A Monoclonal antibody (67936-1-Ig)

    WB analysis of LNCaP using 67936-1-Ig (same clone as 67936-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using SETD1A Monoclonal antibody (67936-1-Ig)

    WB analysis of K-562 using 67936-1-Ig (same clone as 67936-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using SETD1A Monoclonal antibody (67936-1-Ig)

    WB analysis of HSC-T6 using 67936-1-Ig (same clone as 67936-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using SETD1A Monoclonal antibody (67936-1-Ig)

    WB analysis of NIH/3T3 using 67936-1-Ig (same clone as 67936-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67936-1-PBS targets SETD1A in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SETD1A fusion protein Ag30424 相同性解析による交差性が予測される生物種
Full Name SET domain containing 1A
Calculated molecular weight 186 kDa
Observed molecular weight270 kDa
GenBank accession numberNM_014712
Gene symbol SETD1A
Gene ID (NCBI) 9739
RRIDAB_2918688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SETD1A is a SET domain-containing methyltransferase involved in the epigenetic regulation of transcription(PMID:12670868). It is the main catalytic component of a multiprotein complex that methylates lysine 4 of histone H3, a histone mark associated with gene activation (PMID:29937342). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (PMID:31197650). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed:12670868). SETD1A has a calculated molecular weight of 186 kDa, and a 258 kDa band was also reported (PMID: 31076518).