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  • Featured Product
  • KD/KO Validated

SETD8 Polyclonal antibody, PBS Only

SETD8 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14063-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SETD8 Polyclonal antibody (14063-1-AP)

    WB analysis using 14063-1-AP (same clone as 14063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14063-1-AP (SETD8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using SETD8 Polyclonal antibody (14063-1-AP)

    WB analysis of RAW 264.7 using 14063-1-AP (same clone as 14063-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 14063-1-AP (SETD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SETD8 Polyclonal antibody (14063-1-AP)

    IHC staining of human breast cancer using 14063-1-AP (same clone as 14063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14063-1-AP (SETD8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SETD8 Polyclonal antibody (14063-1-AP)

    IHC staining of human breast cancer using 14063-1-AP (same clone as 14063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14063-1-AP (SETD8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293T cells using SETD8 Polyclonal antibody (14063-1-AP)

    FC experiment of HEK-293T using 14063-1-AP (same clone as 14063-1-PBS)

    1x10^6 HEK-293T cells were intracellularly stained with 0.4 ug SETD8 Polyclonal antibody (14063-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14063-1-PBS targets SETD8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SETD8 fusion protein Ag5114 相同性解析による交差性が予測される生物種
Full Name SET domain containing (lysine methyltransferase) 8
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC050346
Gene symbol SETD8
Gene ID (NCBI) 387893
RRIDAB_2878006
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SETD8, also known as Set8, PR-Set7, or KMT5A, is a member of the SET domain family of histone lysine methyltransferases and, to date, the only known enzyme that catalyzes monomethylation of H4K20 in eukaryotes, which is involved in DNA damage response, mitotic condensation, and DNA replication. SETD8 is overexpressed in various types of tumors, such as bladder cancer, non-small cell lung carcinoma, and small cell lung carcinoma, and is associated with a shorter survival time for gastric, esophageal, and prostate cancer patients.