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SETDB1 Polyclonal antibody
SETDB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 11231-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, human testis tissue, MCF-7 cells, PC-3 cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human lung cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
Positive FC (Intra) detected in | A431 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 21 publications below |
WB | See 62 publications below |
IHC | See 7 publications below |
IF | See 11 publications below |
IP | See 9 publications below |
CoIP | See 4 publications below |
ChIP | See 22 publications below |
Product Information
11231-1-AP targets SETDB1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SETDB1 fusion protein Ag1725 相同性解析による交差性が予測される生物種 |
Full Name | SET domain, bifurcated 1 |
Calculated molecular weight | 143 kDa |
Observed molecular weight | 170 kDa |
GenBank accession number | BC009362 |
Gene symbol | SETDB1 |
Gene ID (NCBI) | 9869 |
RRID | AB_2186069 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).
Protocols
Product Specific Protocols | |
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WB protocol for SETDB1 antibody 11231-1-AP | Download protocol |
IHC protocol for SETDB1 antibody 11231-1-AP | Download protocol |
IF protocol for SETDB1 antibody 11231-1-AP | Download protocol |
IP protocol for SETDB1 antibody 11231-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
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Nature Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity.
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Nature Genome surveillance by HUSH-mediated silencing of intronless mobile elements.
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Science GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Hilda (Verified Customer) (07-29-2024) | SETDB1 antibody works very well with human and mouse cell lines for both western blot and ChIP. It requires a short incubation period and produces distinct bands, with a few nonspecific bands observed variable to the type of cells used. CHIP outcomes are still preliminary, but it has resulted in good binding at 2ug antibody used.
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FH Roy (Verified Customer) (06-28-2024) | Does work well to detect SETDB1 in Jurkat and HeLa cells (Overnight incubation at 4°C, 1/1000 dilution)
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FH Taku (Verified Customer) (06-15-2022) |
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