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  • KD/KO Validated

SETDB1 Polyclonal antibody

SETDB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA
Cat No. 11231-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Histone-lysine N-methyltransferase SETDB1, Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, human testis tissue, MCF-7 cells, PC-3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Positive ChIP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
CHImmunoprecipitation (IP)CHIP : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11231-1-AP targets SETDB1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SETDB1 fusion protein Ag1725 相同性解析による交差性が予測される生物種
Full Name SET domain, bifurcated 1
Calculated molecular weight 143 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC009362
Gene Symbol SETDB1
Gene ID (NCBI) 9869
RRIDAB_2186069
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15047
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).

Protocols

Product Specific Protocols
WB protocol for SETDB1 antibody 11231-1-APDownload protocol
IHC protocol for SETDB1 antibody 11231-1-APDownload protocol
IF protocol for SETDB1 antibody 11231-1-APDownload protocol
IP protocol for SETDB1 antibody 11231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Authors - Wenqi Xu
  • KO Validated
mouseWB,CoIP

Nature

The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.

Authors - Jiadong Liu
mouse,humanWB

Nature

Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity.

Authors - Gabriel K Griffin
  • KO Validated
mouseWB,CoIP,ChIP

Nature

KDM5B promotes immune evasion by recruiting SETDB1 to silence retroelements

Authors - Shang-Min Zhang
humanWB

Nature

Genome surveillance by HUSH-mediated silencing of intronless mobile elements.

Authors - Marta Seczynska
  • KD Validated
humanWB

Science

GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.

Authors - Iva A Tchasovnikarova
  • KD Validated
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