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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SETDB1 Monoclonal antibody (66293-1-Ig)

    WB analysis using 66293-1-Ig (same clone as 66293-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66293-1-Ig (SETDB1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using SETDB1 Monoclonal antibody (66293-1-Ig)

    WB analysis of human heart using 66293-1-Ig (same clone as 66293-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66293-1-Ig (SETDB1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SETDB1 Monoclonal antibody (66293-1-Ig)

    WB analysis of HEK-293 using 66293-1-Ig (same clone as 66293-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66293-1-Ig (SETDB1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using SETDB1 Monoclonal antibody (66293-1-Ig)

    IHC staining of mouse colon using 66293-1-Ig (same clone as 66293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using SETDB1 Monoclonal antibody (66293-1-Ig)

    IHC staining of human colon using 66293-1-Ig (same clone as 66293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using SETDB1 Monoclonal antibody (66293-1-Ig)

    IHC staining of human colon using 66293-1-Ig (same clone as 66293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using SETDB1 Monoclonal antibody (66293-1-Ig)

    IHC staining of human colon using 66293-1-Ig (same clone as 66293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using SETDB1 Monoclonal antibody (66293-1-Ig)

    IHC staining of rat liver using 66293-1-Ig (same clone as 66293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using SETDB1 Monoclonal antibody (66293-1-Ig)

    IHC staining of rat colon using 66293-1-Ig (same clone as 66293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SETDB1 Monoclonal antibody (66293-1-Ig)

    IF Staining of A431 using 66293-1-Ig (same clone as 66293-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SETDB1 antibody (66293-1-Ig, Clone: 1H6E5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

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SETDB1 Monoclonal antibody, PBS Only

SETDB1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66293-1-PBS
Clone No.1H6E5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain, EC:2.1.1.366

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66293-1-PBS targets SETDB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SETDB1 fusion protein Ag21644 相同性解析による交差性が予測される生物種
Full Name SET domain, bifurcated 1
Calculated molecular weight 143 kDa
Observed molecular weight 170-180 kDa
GenBank accession numberBC009362
Gene Symbol SETDB1
Gene ID (NCBI) 9869
RRIDAB_2881676
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15047
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).