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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SF1 Polyclonal antibody (11635-1-AP)

    WB analysis using 11635-1-AP (same clone as 11635-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11635-1-AP (SF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using SF1 Polyclonal antibody (11635-1-AP)

    IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using SF1 Polyclonal antibody (11635-1-AP)

    IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11635-1-PBS targets SF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SF1 fusion protein Ag2209 相同性解析による交差性が予測される生物種
Full Name splicing factor 1
Calculated molecular weight 638 aa, 68 kDa
Observed molecular weight65-71 kDa
GenBank accession numberBC020217
Gene Symbol SF1
Gene ID (NCBI) 7536
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15637
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SF1 is a nuclear pre-mRNA splicing factor, it specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The molecular weight of SF1 ranges from 59 kDa to 71 kDa due to alternative splicing.