Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 15858-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 15858-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15858-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 15858-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15858-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15858-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 15858-1-AP
IP result of anti-SF3A1 (IP:15858-1-AP, 4ug; Detection:15858-1-AP 1:500) with mouse brain tissue lysate 4000ug.
IHC staining of human breast cancer using 15858-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:400 (under 10x lens).
IHC staining of human breast cancer using 15858-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:400 (under 40x lens).
IHC staining of human breast cancer using 15858-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human breast cancer using 15858-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 15858-1-AP
Immunofluorescent analysis of HepG2 cells, using SF3A1 antibody 15858-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

SF3A1 Polyclonal antibody

SF3A1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15858-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Spliceosome-associated protein 114, SF3a120, SAP114, SAP 114, PRP21

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HL-60 cells, mouse brain tissue, HEK-293 cells, HeLa cells, mouse lung tissue, K-562 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IP	See 1 publications below

Product Information

15858-1-AP targets SF3A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SF3A1 fusion protein Ag8196 相同性解析による交差性が予測される生物種
Full Name	splicing factor 3a, subunit 1, 120kDa
Calculated molecular weight	793 aa, 89 kDa
Observed molecular weight	120-130 kDa
GenBank accession number	BC007684
Gene Symbol	SF3A1
Gene ID (NCBI)	10291
RRID	AB_2186354
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15459
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )

Protocols

Product Specific Protocols
WB protocol for SF3A1 antibody 15858-1-AP	Download protocol
IHC protocol for SF3A1 antibody 15858-1-AP	Download protocol
IF protocol for SF3A1 antibody 15858-1-AP	Download protocol
IP protocol for SF3A1 antibody 15858-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Rep SF3B1 Association with Chromatin Determines Splicing Outcomes. Authors - Nir Kfir View Article
human	WB	bioRxiv Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations Authors - Sihang Zhou View Article
		Cell Rep Integrative identification of non-coding regulatory regions driving metastatic prostate cancer Authors - Brian J Woo View Article
human	WB	Mol Cell ZC3H14 facilitates backsplicing by binding to exon-intron boundary and 3' UTR Authors - Qiqi Li View Article
mouse	WB	Front Pharmacol LXR agonist inhibits inflammation through regulating MyD88 mRNA alternative splicing Authors - Ni Li View Article
human	IP	bioRxiv Integrative identification of non-coding regulatory regions driving metastatic prostate cancer Authors - Brian J Woo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15858-1-AP

WB analysis of mouse lung using 15858-1-AP

WB analysis of mouse brain using 15858-1-AP

WB analysis of HL-60 using 15858-1-AP

WB analysis of HEK-293 using 15858-1-AP

WB analysis of HeLa using 15858-1-AP

IP experiment of mouse brain using 15858-1-AP

IHC staining of human breast cancer using 15858-1-AP

IHC staining of human breast cancer using 15858-1-AP

IHC staining of human breast cancer using 15858-1-AP

IHC staining of human breast cancer using 15858-1-AP

IF Staining of HepG2 using 15858-1-AP

SF3A1 Polyclonal antibody

SF3A1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SF3B1 Association with Chromatin Determines Splicing Outcomes.

Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations

Integrative identification of non-coding regulatory regions driving metastatic prostate cancer

ZC3H14 facilitates backsplicing by binding to exon-intron boundary and 3' UTR

LXR agonist inhibits inflammation through regulating MyD88 mRNA alternative splicing

Integrative identification of non-coding regulatory regions driving metastatic prostate cancer