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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using SF3A2 Polyclonal antibody (15596-1-AP)

    WB analysis of NIH/3T3 using 15596-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15596-1-AP (SF3A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SF3A2 Polyclonal antibody (15596-1-AP)

    WB analysis of HeLa using 15596-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15596-1-AP (SF3A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SF3A2 Polyclonal antibody (15596-1-AP)

    WB analysis of HeLa using 15596-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15596-1-AP (SF3A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using SF3A2 Polyclonal antibody (15596-1-AP)

    IHC staining of human endometrial cancer using 15596-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15596-1-AP (SF3A2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using SF3A2 Polyclonal antibody (15596-1-AP)

    IHC staining of human endometrial cancer using 15596-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15596-1-AP (SF3A2 antibody) at dilution of 1:50 (under 10x lens).

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SF3A2 Polyclonal antibody

SF3A2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15596-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Synonyms

PRP11, PRPF11, SAP 62, SAP62, SF3A2, SF3a66, Splicing factor 3A subunit 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15596-1-AP targets SF3A2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SF3A2 fusion protein Ag7953 相同性解析による交差性が予測される生物種
Full Name splicing factor 3a, subunit 2, 66kDa
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC004434
Gene Symbol SF3A2
Gene ID (NCBI) 8175
RRIDAB_2301609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15428
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SF3A2 antibody 15596-1-APDownload protocol
IHC protocol for SF3A2 antibody 15596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

J Biol Chem

Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.

Authors - Zhe Chen
View Article
ratWB,IF,CoIP

J Adv Res

Ginsenoside Rb2 inhibits p300-mediated SF3A2 acetylation at lysine 10 to promote Fscn1 alternative splicing against myocardial ischemic/reperfusion injury

Authors - Qingxia Huang
View Article