Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 12070-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12070-1-AP
WB result of SF3A3 antibody (12070-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SF3A3 transfected A549 cells.
WB analysis of Raji using 12070-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 12070-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 12070-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 12070-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 12070-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 12070-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 12070-1-AP
IP result of anti-SF3A3 (IP:12070-1-AP, 4ug; Detection:12070-1-AP 1:600) with Raji cells lysate 2000ug.
IHC staining of human lung cancer using 12070-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12070-1-AP (SF3A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12070-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12070-1-AP (SF3A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12070-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12070-1-AP (SF3A3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 12070-1-AP
Immunofluorescent analysis of Hela cells, using SF3A3 antibody 12070-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

SF3A3 Polyclonal antibody

SF3A3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12070-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Spliceosome-associated protein 61, SF3a60, SAP61, SAP 61, PRP9

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, Raji cells, A549 cells, K-562 cells, NIH/3T3 cells
Positive IP detected in	Raji cells
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

12070-1-AP targets SF3A3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SF3A3 fusion protein Ag2708 相同性解析による交差性が予測される生物種
Full Name	splicing factor 3a, subunit 3, 60kDa
Calculated molecular weight	501 aa, 59 kDa
Observed molecular weight	59 kDa
GenBank accession number	BC011523
Gene Symbol	SF3A3
Gene ID (NCBI)	10946
RRID	AB_2186372
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q12874
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SF3A3 antibody 12070-1-AP	Download protocol
IHC protocol for SF3A3 antibody 12070-1-AP	Download protocol
IF protocol for SF3A3 antibody 12070-1-AP	Download protocol
IP protocol for SF3A3 antibody 12070-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	bioRxiv Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations Authors - Sihang Zhou View Article
bovine	WB	Int J Mol Sci Autophagy Mediates Escherichia Coli-Induced Cellular Inflammatory Injury by Regulating Calcium Mobilization, Mitochondrial Dysfunction, and Endoplasmic Reticulum Stress Authors - Jianguo Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12070-1-AP

WB analysis of A549 using 12070-1-AP

WB analysis of Raji using 12070-1-AP

WB analysis of Raji using 12070-1-AP

WB analysis of Raji using 12070-1-AP

WB analysis of Raji using 12070-1-AP

WB analysis of Raji using 12070-1-AP

WB analysis using 12070-1-AP

IP experiment of Raji using 12070-1-AP

IHC staining of human lung cancer using 12070-1-AP

IHC staining of human lung cancer using 12070-1-AP

IF Staining of HeLa using 12070-1-AP

IF Staining of HeLa using 12070-1-AP

SF3A3 Polyclonal antibody

SF3A3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations

Autophagy Mediates Escherichia Coli-Induced Cellular Inflammatory Injury by Regulating Calcium Mobilization, Mitochondrial Dysfunction, and Endoplasmic Reticulum Stress