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SF3B1 Polyclonal antibody
SF3B1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 27684-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, HeLa cells, U2OS cells |
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 5 publications below |
Product Information
27684-1-AP targets SF3B1 in WB, IHC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SF3B1 fusion protein Ag26689 相同性解析による交差性が予測される生物種 |
Full Name | splicing factor 3b, subunit 1, 155kDa |
Calculated molecular weight | 146 kDa |
Observed molecular weight | 146-150 kDa |
GenBank accession number | NM_012433 |
Gene symbol | SF3B1 |
Gene ID (NCBI) | 23451 |
RRID | AB_2880946 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Protocols
Product Specific Protocols | |
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WB protocol for SF3B1 antibody 27684-1-AP | Download protocol |
IHC protocol for SF3B1 antibody 27684-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Gastroenterology Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p. | ||
EMBO Rep A splicing factor switch controls hematopoietic lineage specification of pluripotent stem cells.
| ||
Sci Rep Exploring the mechanistic link between SF3B1 mutation and ring sideroblast formation in myelodysplastic syndrome | ||
Nat Commun Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12 | ||
EMBO J A transcription-independent mechanism determines rapid periodic fluctuations of BRCA1 expression |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Wael (Verified Customer) (09-29-2022) | specific band between 100 and 150 kd
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