Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, HeLa cells, U2OS cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
Product Information
27684-1-AP targets SF3B1 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SF3B1 fusion protein Ag26689 相同性解析による交差性が予測される生物種 |
| Full Name | splicing factor 3b, subunit 1, 155kDa |
| Calculated molecular weight | 146 kDa |
| Observed molecular weight | 146-150 kDa |
| GenBank accession number | NM_012433 |
| Gene Symbol | SF3B1 |
| Gene ID (NCBI) | 23451 |
| RRID | AB_2880946 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75533 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SF3B1 antibody 27684-1-AP | Download protocol |
| IHC protocol for SF3B1 antibody 27684-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gastroenterology Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p. | ||
EMBO Rep A splicing factor switch controls hematopoietic lineage specification of pluripotent stem cells.
| ||
Sci Rep Exploring the mechanistic link between SF3B1 mutation and ring sideroblast formation in myelodysplastic syndrome | ||
Nat Commun Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12 | ||
EMBO J A transcription-independent mechanism determines rapid periodic fluctuations of BRCA1 expression | ||
Mol Cell Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles |