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  • KD/KO Validated

SF3B1 Polyclonal antibody

SF3B1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27684-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

PRP10, PRPF10, SAP 155, SAP155, SF3B1, SF3b155, Splicing factor 3B subunit 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, U2OS cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27684-1-AP targets SF3B1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SF3B1 fusion protein Ag26689 相同性解析による交差性が予測される生物種
Full Name splicing factor 3b, subunit 1, 155kDa
Calculated molecular weight 146 kDa
Observed molecular weight 146-150 kDa
GenBank accession numberNM_012433
Gene Symbol SF3B1
Gene ID (NCBI) 23451
RRIDAB_2880946
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Protocols

Product Specific Protocols
WB protocol for SF3B1 antibody 27684-1-APDownload protocol
IHC protocol for SF3B1 antibody 27684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gastroenterology

Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p.

Authors - Yingying Cao
humanWB

EMBO Rep

A splicing factor switch controls hematopoietic lineage specification of pluripotent stem cells.

Authors - Yapu Li
  • KD Validated
humanWB

Sci Rep

Exploring the mechanistic link between SF3B1 mutation and ring sideroblast formation in myelodysplastic syndrome

Authors - Tetsuro Ochi
humanWB

Nat Commun

Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12

Authors - Zixiang Wang
humanWB

EMBO J

A transcription-independent mechanism determines rapid periodic fluctuations of BRCA1 expression

Authors - Chen Nie
humanWB

Mol Cell

Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles

Authors - Wael Kamel
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