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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SF3B5 Polyclonal antibody

SF3B5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15525-1-AP

Synonyms

Splicing factor 3B subunit 5, SF3B10, Pre-mRNA-splicing factor SF3b 10 kDa subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using SF3B5 Polyclonal antibody (15525-1-AP)

    WB analysis of SH-SY5Y using 15525-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15525-1-AP (SF3B5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SF3B5 Polyclonal antibody (15525-1-AP)

    WB analysis of HeLa using 15525-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15525-1-AP (SF3B5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using SF3B5 Polyclonal antibody (15525-1-AP)

    WB analysis of mouse eye using 15525-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 15525-1-AP (SF3B5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SF3B5 Polyclonal antibody (15525-1-AP)

    IHC staining of human ovary tumor using 15525-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15525-1-AP (SF3B5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SF3B5 Polyclonal antibody (15525-1-AP)

    IHC staining of human ovary tumor using 15525-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15525-1-AP (SF3B5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SF3B5 Polyclonal antibody (15525-1-AP)

    IF Staining of HeLa using 15525-1-AP

    Immunofluorescent analysis of Hela cells, using SF3B5 antibody 15525-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inSH-SY5Y cells, HeLa cells, mouse eye tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15525-1-AP targets SF3B5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SF3B5 fusion protein Ag7205 相同性解析による交差性が予測される生物種
Full Name splicing factor 3b, subunit 5, 10kDa
Calculated molecular weight 10 kDa
Observed molecular weight 10-12 kDa
GenBank accession numberBC000198
Gene symbol SF3B5
Gene ID (NCBI) 83443
RRIDAB_10596628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SF3B5 antibody 15525-1-APDownload protocol
IHC protocol for SF3B5 antibody 15525-1-APDownload protocol
IF protocol for SF3B5 antibody 15525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols