Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, mouse lung tissue |
| Positive IF/ICC detected in | HepG2 cells, Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
13550-1-AP targets SFI1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SFI1 fusion protein Ag4268 相同性解析による交差性が予測される生物種 |
| Full Name | Sfi1 homolog, spindle assembly associated (yeast) |
| Calculated molecular weight | 1211 aa, 134 kDa |
| Observed molecular weight | 148 kDa |
| GenBank accession number | BC033613 |
| Gene Symbol | SFI1 |
| Gene ID (NCBI) | 9814 |
| RRID | AB_10596938 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A8K8P3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SFI1 antibody 13550-1-AP | Download protocol |
| IF protocol for SFI1 antibody 13550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EBioMedicine PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response | ||
J Cell Biol SFI1 promotes centriole duplication by recruiting USP9X to stabilize the microcephaly protein STIL. | ||
J Cell Biol CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity | ||
Sci Signal Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins. | ||
Sci Rep Dietary glucoraphanin prevents the onset of psychosis in the adult offspring after maternal immune activation. | ||
J Cell Sci Differential requirements for the EF-hands of human centrin2 in primary ciliogenesis and nucleotide excision repair. |