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  • Featured Product
  • KD/KO Validated

SFPQ Monoclonal antibody, PBS Only

SFPQ Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G4A5

Cat no : 67129-1-PBS

Synonyms

POMP100, POMP 100, Polypyrimidine tract-binding protein-associated-splicing factor, DNA-binding p52/p100 complex, 100 kDa subunit, 1G4A5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SFPQ Monoclonal antibody (67129-1-Ig)

    WB analysis using 67129-1-Ig (same clone as 67129-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SFPQ Monoclonal antibody (67129-1-Ig)

    WB analysis using 67129-1-Ig (same clone as 67129-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SFPQ Monoclonal antibody (67129-1-Ig)

    WB analysis of HeLa using 67129-1-Ig (same clone as 67129-1-PBS)

    WB result of SFPQ antibody (67129-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFPQ transfected HeLa cells. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SFPQ Monoclonal antibody (67129-1-Ig)

    WB analysis of HeLa using 67129-1-Ig (same clone as 67129-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SFPQ Monoclonal antibody (67129-1-Ig)

    WB analysis of Jurkat using 67129-1-Ig (same clone as 67129-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using SFPQ Monoclonal antibody (67129-1-Ig)

    WB analysis of PC-3 using 67129-1-Ig (same clone as 67129-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SFPQ Monoclonal antibody (67129-1-Ig)

    WB analysis of HeLa using 67129-1-Ig (same clone as 67129-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SFPQ Monoclonal antibody (67129-1-Ig)

    WB analysis of HEK-293 using 67129-1-Ig (same clone as 67129-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SFPQ Monoclonal antibody (67129-1-Ig)

    WB analysis of Jurkat using 67129-1-Ig (same clone as 67129-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using SFPQ Monoclonal antibody (67129-1-Ig)

    WB analysis of HSC-T6 using 67129-1-Ig (same clone as 67129-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using SFPQ Monoclonal antibody (67129-1-Ig)

    WB analysis of NIH/3T3 using 67129-1-Ig (same clone as 67129-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using SFPQ Monoclonal antibody (67129-1-Ig)

    IHC staining of rat stomach using 67129-1-Ig (same clone as 67129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SFPQ Monoclonal antibody (67129-1-Ig)

    IHC staining of human colon cancer using 67129-1-Ig (same clone as 67129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SFPQ Monoclonal antibody (67129-1-Ig)

    IHC staining of human lung cancer using 67129-1-Ig (same clone as 67129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SFPQ Monoclonal antibody (67129-1-Ig)

    IHC staining of human pancreas cancer using 67129-1-Ig (same clone as 67129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SFPQ Monoclonal antibody (67129-1-Ig)

    IHC staining of mouse brain using 67129-1-Ig (same clone as 67129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using SFPQ Monoclonal antibody (67129-1-Ig)

    IHC staining of mouse stomach using 67129-1-Ig (same clone as 67129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SFPQ Monoclonal antibody (67129-1-Ig)

    IHC staining of rat brain using 67129-1-Ig (same clone as 67129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SFPQ Monoclonal antibody (67129-1-Ig)

    IF Staining of MCF-7 using 67129-1-Ig (same clone as 67129-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SFPQ antibody (67129-1-Ig, Clone: 1G4A5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SFPQ Monoclonal antibody (67129-1-Ig)

    IF Staining of HeLa using 67129-1-Ig (same clone as 67129-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SFPQ antibody (67129-1-Ig, Clone: 1G4A5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SFPQ Monoclonal antibody (67129-1-Ig)

    FC experiment of HeLa using 67129-1-Ig (same clone as 67129-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SFPQ (67129-1-Ig, Clone:1G4A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67129-1-PBS targets SFPQ in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SFPQ fusion protein Ag7181 相同性解析による交差性が予測される生物種
Full Name splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated)
Calculated molecular weight 76 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC051192
Gene symbol SFPQ
Gene ID (NCBI) 6421
RRIDAB_2882428
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SFPQ, also named PSF, encodes a nuclear factor implicated in the splicing and regulation of gene expression. SFPQ probably forms a heteromer with NONO and participates in DNA pairing and DNA break repair program. Very recently SFPQ was identified as a downstream target of tau, complete nuclear depletion and cytoplasmic accumulation of SFPQ were shown in the neurons and astrocytes of brains with Alzheimer's disease (AD), more strikingly, reduced SFPQ levels may progress together with tau pathology, these observation strongly suggests the important role of SFPQ pathology in neurodegenerative diseases including AD. SFPQ encompasses 707 amino acids and has a molecular weight of 76 kDa, although it typically migrates on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel at an apparent molecular weight of ∼100 kDa. Proteolytic cleavage products of apparent molecular weights of 47 and 68 kDa, and an alternatively spliced form of 669 amino acids, have also been described in various cell types. (PMID: 25832716). Splicing Factor Proline and Glutamine rich (SFPQ) as the most significant intron-retaining transcript across diverse ALS-causing mutations (VCP, SOD1 and FUS). SFPQ protein binds extensively to its retained intron, which exhibits high cytoplasmic abundance in VCP mutation compared with controls. Crucially, the protein is less abundant in the nuclei of VCP mutation cultures and is ultimately lost from nuclei of MNs in mouse models (SOD1mu and VCP mutation transgenic mouse models) and human sporadic ALS post-mortem samples. In summary, our study implicates SFPQ IR and nuclear loss as general molecular hallmarks of familial and sporadic ALS.