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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SFRP4 Polyclonal antibody (15328-1-AP)

    WB analysis of HEK-293 using 15328-1-AP (same clone as 15328-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15328-1-AP (SFRP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SFRP4 Polyclonal antibody (15328-1-AP)

    WB analysis of HeLa using 15328-1-AP (same clone as 15328-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15328-1-AP (SFRP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SFRP4 Polyclonal antibody (15328-1-AP)

    IP experiment of HeLa using 15328-1-AP (same clone as 15328-1-PBS)

    IP result of anti-SFRP4 (IP:15328-1-AP, 4ug; Detection:15328-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SFRP4 Polyclonal antibody (15328-1-AP)

    IHC staining of human breast cancer using 15328-1-AP (same clone as 15328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SFRP4 Polyclonal antibody (15328-1-AP)

    IHC staining of human breast cancer using 15328-1-AP (same clone as 15328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using SFRP4 Polyclonal antibody (15328-1-AP)

    IHC staining of rat testis using 15328-1-AP (same clone as 15328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SFRP4 Polyclonal antibody (15328-1-AP)

    IHC staining of human skin cancer using 15328-1-AP (same clone as 15328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SFRP4 Polyclonal antibody (15328-1-AP)

    IHC staining of human skin cancer using 15328-1-AP (same clone as 15328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SFRP4 Polyclonal antibody (15328-1-AP)

    IHC staining of human skin cancer using 15328-1-AP (same clone as 15328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SFRP4 Polyclonal antibody (15328-1-AP)

    IHC staining of human skin cancer using 15328-1-AP (same clone as 15328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SFRP4 Polyclonal antibody, PBS Only

SFRP4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15328-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15328-1-PBS targets SFRP4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SFRP4 fusion protein Ag6909 相同性解析による交差性が予測される生物種
Full Name secreted frizzled-related protein 4
Calculated molecular weight 40 kDa
Observed molecular weight 41-51 kDa, 55 kDa
GenBank accession numberBC058911
Gene Symbol SFRP4
Gene ID (NCBI) 6424
RRIDAB_2187099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6FHJ7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRP4 functions as a suppressor of tumor growth via inhibition of the Wnt signaling pathway.Downregulation or deletion of SFRP4 has been reported in several cancers and cell lines such as endometrial cancer, ovarian cancer, lung cancer,and others. This antibody got 41-51 kDa and 55 kDa bands in western blotting maybe due to differential posttranslational protein modification.