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  • Featured Product
  • KD/KO Validated

SFRS11 Polyclonal antibody

SFRS11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26751-1-AP

Synonyms

p54, SFRS11, SRSF11

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using SFRS11 Polyclonal antibody (26751-1-AP)

    WB analysis of HEK-293T using 26751-1-AP

    WB result of SFRS11 antibody (26751-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFRS11 transfected HEK-293T cells.

  • Western Blot (WB) analysis of various lysates using SFRS11 Polyclonal antibody (26751-1-AP)

    WB analysis using 26751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26751-1-AP (SFRS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SFRS11 Polyclonal antibody (26751-1-AP)

    IHC staining of human breast cancer using 26751-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SFRS11 Polyclonal antibody (26751-1-AP)

    IHC staining of human breast cancer using 26751-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat colon tissue using SFRS11 Polyclonal antibody (26751-1-AP)

    IHC staining of rat colon using 26751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using SFRS11 Polyclonal antibody (26751-1-AP)

    IHC staining of rat colon using 26751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293T cells
Positive IHC detected inhuman breast cancer tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26751-1-AP targets SFRS11 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SFRS11 fusion protein Ag25015 相同性解析による交差性が予測される生物種
Full Name splicing factor, arginine/serine-rich 11
Calculated molecular weight 483 aa, 53 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC040436
Gene symbol SFRS11
Gene ID (NCBI) 9295
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SFRS11 antibody 26751-1-APDownload protocol
IHC protocol for SFRS11 antibody 26751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols