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  • Featured Product
  • KD/KO Validated

SFRS11 Polyclonal antibody

SFRS11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26751-1-AP

Synonyms

p54, SFRS11, SRSF11

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using SFRS11 Polyclonal antibody (26751-1-AP)

    WB analysis of HEK-293T using 26751-1-AP

    WB result of SFRS11 antibody (26751-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFRS11 transfected HEK-293T cells.

  • Western Blot (WB) analysis of various lysates using SFRS11 Polyclonal antibody (26751-1-AP)

    WB analysis using 26751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26751-1-AP (SFRS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SFRS11 Polyclonal antibody (26751-1-AP)

    IHC staining of human breast cancer using 26751-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SFRS11 Polyclonal antibody (26751-1-AP)

    IHC staining of human breast cancer using 26751-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat colon tissue using SFRS11 Polyclonal antibody (26751-1-AP)

    IHC staining of rat colon using 26751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using SFRS11 Polyclonal antibody (26751-1-AP)

    IHC staining of rat colon using 26751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293T cells
Positive IHC detected inhuman breast cancer tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26751-1-AP targets SFRS11 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SFRS11 fusion protein Ag25015 相同性解析による交差性が予測される生物種
Full Name splicing factor, arginine/serine-rich 11
Calculated molecular weight 483 aa, 53 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC040436
Gene symbol SFRS11
Gene ID (NCBI) 9295
RRIDAB_3085902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SFRS11 antibody 26751-1-APDownload protocol
IHC protocol for SFRS11 antibody 26751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols