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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SFRS11 Recombinant antibody (83605-6-RR)

    WB analysis using 83605-6-RR (same clone as 83605-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83605-6-RR (SFRS11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83605-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SFRS11 Recombinant antibody (83605-6-RR)

    IF Staining of HepG2 using 83605-6-RR (same clone as 83605-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SFRS11 antibody (83605-6-RR, Clone: 240465B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83605-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SFRS11 Recombinant antibody (83605-6-RR)

    IF Staining of HepG2 using 83605-6-RR (same clone as 83605-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SFRS11 antibody (83605-6-RR, Clone: 240465B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83605-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SFRS11 Recombinant antibody (83605-6-RR)

    FC experiment of HeLa using 83605-6-RR (same clone as 83605-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SFRS11 Recombinant antibody (83605-6-RR, Clone:240465B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83605-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83605-6-RR

    Affinity and Kinetic Characterization of 83605-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83605-6-RR against Human SFRS11 were performed. The affinity constant is 1.37 nM.

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SFRS11 Recombinant antibody, PBS Only

SFRS11 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83605-6-PBS
Clone No.240465B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

SRSF11, Splicing factor, arginine/serine-rich 11, Serine/arginine-rich splicing factor 11, p54, Arginine-rich 54 kDa nuclear protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83605-6-PBS targets SFRS11 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SFRS11 fusion protein Ag24932 相同性解析による交差性が予測される生物種
Full Name splicing factor, arginine/serine-rich 11
Calculated molecular weight 483 aa, 53 kDa
GenBank accession numberBC040436
Gene Symbol SFRS11
Gene ID (NCBI) 9295
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ05519
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.