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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SFRS17A Polyclonal antibody (13441-1-AP)

    WB analysis of HeLa using 13441-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SFRS17A Polyclonal antibody (13441-1-AP)

    WB analysis of Jurkat using 13441-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using SFRS17A Polyclonal antibody (13441-1-AP)

    WB analysis of HEK-293T using 13441-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using SFRS17A Polyclonal antibody (13441-1-AP)

    WB analysis of human kidney using 13441-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using SFRS17A Polyclonal antibody (13441-1-AP)

    WB analysis of human heart using 13441-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using SFRS17A Polyclonal antibody (13441-1-AP)

    IHC staining of mouse cerebellum using 13441-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13441-1-AP (SFRS17A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using SFRS17A Polyclonal antibody (13441-1-AP)

    IHC staining of mouse cerebellum using 13441-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13441-1-AP (SFRS17A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SFRS17A Polyclonal antibody (13441-1-AP)

    IHC staining of human pancreas cancer using 13441-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13441-1-AP (SFRS17A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SFRS17A Polyclonal antibody (13441-1-AP)

    IHC staining of human pancreas cancer using 13441-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13441-1-AP (SFRS17A antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HT-1080 cells using SFRS17A Polyclonal antibody (13441-1-AP)

    IF Staining of HT-1080 using 13441-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HT-1080 cells using SFRS17A antibody (13441-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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SFRS17A Polyclonal antibody

SFRS17A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13441-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CXYorf3, B-lymphocyte antigen, A-kinase anchor protein 17A, AKAP-17A, AKAP17A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, human heart tissue, human kidney tissue, HEK-293T cells
Positive IHC detected inmouse cerebellum tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHT-1080 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13441-1-AP targets SFRS17A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SFRS17A fusion protein Ag4252 相同性解析による交差性が予測される生物種
Full Name splicing factor, arginine/serine-rich 17A
Calculated molecular weight 695 aa, 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC028151
Gene Symbol SFRS17A
Gene ID (NCBI) 8227
RRIDAB_2184937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02040
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SFRS17A antibody 13441-1-APDownload protocol
IHC protocol for SFRS17A antibody 13441-1-APDownload protocol
IF protocol for SFRS17A antibody 13441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols