Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, mouse testis tissue, A549 cells, rat brain tissue, HepG2 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1500-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 10 publications below |
| IF | See 4 publications below |
| RIP | See 1 publications below |
Product Information
20371-1-AP targets SFRS2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | splicing factor, arginine/serine-rich 2 |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 25-35 kDa |
| GenBank accession number | NM_003016 |
| Gene Symbol | SFRS2 |
| Gene ID (NCBI) | 6427 |
| RRID | AB_2616311 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01130 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SFRS2, also named as PR264 and Splicing component, 35 kDa, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. Some residue of SFRS2 is modified by phosphorylase and acetylase after translation. The molecular weight of modified SFRS2 is 35-40kDa. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. The antibody has no cross reaction to SFRS2B.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SFRS2 antibody 20371-1-AP | Download protocol |
| IHC protocol for SFRS2 antibody 20371-1-AP | Download protocol |
| IF protocol for SFRS2 antibody 20371-1-AP | Download protocol |
| IP protocol for SFRS2 antibody 20371-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth. | ||
EMBO Mol Med SRSF protein kinase 1 modulates RAN translation and suppresses CGG repeat toxicity | ||
Am J Cancer Res tRF-20-M0NK5Y93-induced MALAT1 promotes colon cancer metastasis through alternative splicing of SMC1A
| ||
J Biol Chem Proteomic study identifies Aurora-A mediated regulation of alternative splicing through multiple splicing factors | ||
Neuroscience The Age-dependent Elevation of miR-335-3p Leads to Reduced Cholesterol and Impaired Memory in Brain. | ||
Cell Rep SFRS11 Loss Leads to Aging-Associated Cognitive Decline by Modulating LRP8 and ApoE. |