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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SFRS2 Polyclonal antibody

SFRS2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21212-1-AP

Synonyms

Serine/arginine-rich splicing factor 2, SC-35, SC35, SC 35, Protein PR264

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SFRS2 Polyclonal antibody (21212-1-AP)

    WB analysis using 21212-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21212-1-AP (SFRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21212-1-AP targets SFRS2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SFRS2 fusion protein Ag15612 相同性解析による交差性が予測される生物種
Full Name splicing factor, arginine/serine-rich 2
Calculated molecular weight 221 aa, 25 kDa
Observed molecular weight30 kDa
GenBank accession numberBC070086
Gene symbol SFRS2
Gene ID (NCBI) 6427
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SFRS2, also named as PR264 and SC-35, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. The antibody has no cross reaction to SFRS2B.

Protocols

Product Specific Protocols
WB protocol for SFRS2 antibody 21212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols