SGCG Polyclonal antibody

Name: SGCG Polyclonal antibody
Brand: Proteintech
SKU: 18102-1-AP

SGCG Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18102-1-AP

Synonyms

35DAG, A4, DAGA4, DMDA, DMDA1, Gamma sarcoglycan, Gamma SG, LGMD2C, MAM, SCARMD2, SCG3, SGCG, TYPE

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis using 18102-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 18102-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 18102-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 18102-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 18102-1-AP
IP result of anti-SGCG (IP:18102-1-AP, 4ug; Detection:18102-1-AP 1:1000) with mouse heart tissue lysate 4000ug.
IHC staining of human heart using 18102-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 18102-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 18102-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 18102-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 18102-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 18102-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 18102-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 18102-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 18102-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 18102-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary using 18102-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 18102-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 18102-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse heart tissue, human skeletal muscle tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 2 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

18102-1-AP targets SGCG in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SGCG fusion protein Ag12402 相同性解析による交差性が予測される生物種
Full Name	sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
Calculated molecular weight	291 aa, 32 kDa
Observed molecular weight	35-38 kDa
GenBank accession number	BC074777
Gene symbol	SGCG
Gene ID (NCBI)	6445
RRID	AB_2286186
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SGCG antibody 18102-1-AP	Download protocol
IHC protocol for SGCG antibody 18102-1-AP	Download protocol
IP protocol for SGCG antibody 18102-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF,IP	Skelet Muscle Novel γ-sarcoglycan interactors in murine muscle membranes. Authors - Tara C Smith View Article
mouse	IHC	PLoS One Peptide-conjugated phosphodiamidate oligomer-mediated exon skipping has benefits for cardiac function in mdx and Cmah-/-mdx mouse models of Duchenne muscular dystrophy. Authors - Alison M Blain View Article
human	IF	PLoS One Correlation of Utrophin Levels with the Dystrophin Protein Complex and Muscle Fibre Regeneration in Duchenne and Becker Muscular Dystrophy Muscle Biopsies. Authors - Narinder Janghra View Article
human	IF	Hum Gene Ther Methods Optimisation of internally deleted dystrophin constructs. Authors - Mojgan Reza View Article
mouse	IHC	Mol Vis Identification of HMX1 target genes: a predictive promoter model approach. Authors - Boulling Arnaud A View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SGCG Polyclonal antibody

SGCG Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 18102-1-AP

WB analysis of human skeletal muscle using 18102-1-AP

WB analysis of human heart using 18102-1-AP

WB analysis of mouse heart using 18102-1-AP

IP experiment of mouse heart using 18102-1-AP

IHC staining of human heart using 18102-1-AP

IHC staining of human heart using 18102-1-AP

IHC staining of human kidney using 18102-1-AP

IHC staining of human kidney using 18102-1-AP

IHC staining of human placenta using 18102-1-AP

IHC staining of human placenta using 18102-1-AP

IHC staining of human skin using 18102-1-AP

IHC staining of human skin using 18102-1-AP

IHC staining of human lung using 18102-1-AP

IHC staining of human lung using 18102-1-AP

IHC staining of human ovary using 18102-1-AP

IHC staining of human ovary using 18102-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Novel γ-sarcoglycan interactors in murine muscle membranes.

Peptide-conjugated phosphodiamidate oligomer-mediated exon skipping has benefits for cardiac function in mdx and Cmah-/-mdx mouse models of Duchenne muscular dystrophy.

Correlation of Utrophin Levels with the Dystrophin Protein Complex and Muscle Fibre Regeneration in Duchenne and Becker Muscular Dystrophy Muscle Biopsies.

Optimisation of internally deleted dystrophin constructs.

Identification of HMX1 target genes: a predictive promoter model approach.