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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SGCZ Polyclonal antibody

SGCZ Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 21614-1-AP

Synonyms

ZSG1, Zeta-SG, Zeta-sarcoglycan, Zeta sarcoglycan

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SGCZ Polyclonal antibody (21614-1-AP)

    WB analysis using 21614-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21614-1-AP (SGCZ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SGCZ Polyclonal antibody (21614-1-AP)

    WB analysis using 21614-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21614-1-AP (SGCZ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using SGCZ Polyclonal antibody (21614-1-AP)

    IF Staining of mouse heart using 21614-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SGCZ antibody (21614-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse heart tissue, mouse brain tissue, rat heart tissue
Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21614-1-AP targets SGCZ in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGCZ fusion protein Ag16305 相同性解析による交差性が予測される生物種
Full Name sarcoglycan zeta
Calculated molecular weight 265 aa, 30 kDa
Observed molecular weight30-40 kDa
GenBank accession numberBC125037
Gene symbol SGCZ
Gene ID (NCBI) 137868
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sarcoglycan zeta, also known as SGCZ, ZSG1, is part of the sarcoglycan complex. The sarcoglycan complex is part of the dystrophin-associated glycoprotein complex (DGC), which bridges the inner cytoskeleton and the extracellular matrix. SGCZ plays a role in the maintenance of striated muscle membrane stability. SGCZ was also found as a component of the vascular smooth muscle sarcoglycan complex. SGCZ was reduced at the membrane in muscular dystrophy (PMID: 12189167).

Protocols

Product Specific Protocols
WB protocol for SGCZ antibody 21614-1-APDownload protocol
IF protocol for SGCZ antibody 21614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols