SGEF Polyclonal antibody
SGEF Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, FC, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12493-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-3 cells, HeLa cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
FC | See 1 publications below |
Product Information
12493-1-AP targets SGEF in WB, FC, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SGEF fusion protein Ag3198 相同性解析による交差性が予測される生物種 |
Full Name | Src homology 3 domain-containing guanine nucleotide exchange factor |
Calculated molecular weight | 97 kDa |
Observed molecular weight | 97 kDa |
GenBank accession number | BC016628 |
Gene symbol | SGEF |
Gene ID (NCBI) | 26084 |
RRID | AB_10896997 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for SGEF antibody 12493-1-AP | Download protocol |
IHC protocol for SGEF antibody 12493-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
PLoS One The guanine-nucleotide exchange factor SGEF plays a crucial role in the formation of atherosclerosis. |