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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SGEF Polyclonal antibody

SGEF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12493-1-AP

Synonyms

CSGEF, HMFN1864, SGEF

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using SGEF Polyclonal antibody (12493-1-AP)

    WB analysis of PC-3 using 12493-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SGEF Polyclonal antibody (12493-1-AP)

    WB analysis of PC-3 using 12493-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SGEF Polyclonal antibody (12493-1-AP)

    WB analysis of HeLa using 12493-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SGEF Polyclonal antibody (12493-1-AP)

    WB analysis of HeLa using 12493-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SGEF Polyclonal antibody (12493-1-AP)

    IHC staining of human prostate cancer using 12493-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12493-1-AP (SGEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SGEF Polyclonal antibody (12493-1-AP)

    IHC staining of human prostate cancer using 12493-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12493-1-AP (SGEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inPC-3 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

12493-1-AP targets SGEF in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGEF fusion protein Ag3198 相同性解析による交差性が予測される生物種
Full Name Src homology 3 domain-containing guanine nucleotide exchange factor
Calculated molecular weight 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC016628
Gene symbol SGEF
Gene ID (NCBI) 26084
RRIDAB_10896997
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SGEF antibody 12493-1-APDownload protocol
IHC protocol for SGEF antibody 12493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

PLoS One

The guanine-nucleotide exchange factor SGEF plays a crucial role in the formation of atherosclerosis.

Authors - Samson Thomas T
View Article