SGF29 Monoclonal antibody
SGF29 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1G1B4
Cat no : 68339-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig liver tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68339-1-Ig targets SGF29 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
Tested Reactivity | Human, Mouse, Rat, Pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | SGF29 fusion protein Ag33219 相同性解析による交差性が予測される生物種 |
Full Name | coiled-coil domain containing 101 |
Calculated molecular weight | 293 aa, 33 kDa |
Observed molecular weight | 33 kDa |
GenBank accession number | BC011981 |
Gene symbol | SGF29 |
Gene ID (NCBI) | 112869 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SAGA-associated factor 29 (SGF29) is a chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes. SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PMID:19103755, PMID:26421618, PMID:21685874, PMID:26578293). Involved in the response to the endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (PubMed:23894581). It has a calculated molecular weight of 33 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for SGF29 antibody 68339-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |