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  • Featured Product
  • KD/KO Validated

SGK1 Polyclonal antibody, PBS Only

SGK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28454-1-PBS

Synonyms

SGK, Serum/glucocorticoid-regulated kinase 1, Serine/threonine-protein kinase Sgk1, EC:2.7.11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SGK1 Polyclonal antibody (28454-1-AP)

    WB analysis using 28454-1-AP (same clone as 28454-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28454-1-AP (SGK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28454-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SGK1 Polyclonal antibody (28454-1-AP)

    IHC staining of mouse kidney using 28454-1-AP (same clone as 28454-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28454-1-AP (SGK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28454-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SGK1 Polyclonal antibody (28454-1-AP)

    IHC staining of mouse kidney using 28454-1-AP (same clone as 28454-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28454-1-AP (SGK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28454-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SGK1 Polyclonal antibody (28454-1-AP)

    IHC staining of human liver cancer using 28454-1-AP (same clone as 28454-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28454-1-AP (SGK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28454-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SGK1 Polyclonal antibody (28454-1-AP)

    IHC staining of human liver cancer using 28454-1-AP (same clone as 28454-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28454-1-AP (SGK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28454-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SGK1 Polyclonal antibody (28454-1-AP)

    IF Staining of HepG2 using 28454-1-AP (same clone as 28454-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SGK1 antibody (28454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28454-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SGK1 Polyclonal antibody (28454-1-AP)

    IF Staining of HepG2 using 28454-1-AP (same clone as 28454-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SGK1 antibody (28454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28454-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using SGK1 Polyclonal antibody (28454-1-AP)

    IF Staining of SH-SY5Y using 28454-1-AP (same clone as 28454-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SGK1 antibody (28454-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28454-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28454-1-PBS targets SGK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGK1 fusion protein Ag29420 相同性解析による交差性が予測される生物種
Full Name serum/glucocorticoid regulated kinase 1
Calculated molecular weight 431 aa, 49 kDa
Observed molecular weight 42-50 kDa
GenBank accession numberBC001263
Gene symbol SGK1
Gene ID (NCBI) 6446
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Serum- and glucocorticoid-induced kinase 1 (SGK1),also named as SGK, is a S/T protein kinase that belongs to the AGC (cAMP-dependent, cGMP-dependent, and protein kinase C) kinase family. It is expressed in many cell types and participates in numerous cellular processes and it is ubiquitinated and degraded at the ER membrane(PMID: 16847254). The N-terminal motif of SGK1 is critical for its ubiquitination and degradation(PMID:16817852 ). SGK and Akt are likely to phosphorylate related substrates, as they share a similar consensus phosphorylation site (RXRXXS/T)(PMID:11154281). It has 5 isoforms produced by alternative promoter usage and alternative splicing.