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  • Featured Product
  • KD/KO Validated

SGK2 Polyclonal antibody

SGK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11185-1-AP

Synonyms

H SGK2, SGK2

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using SGK2 Polyclonal antibody (11185-1-AP)

    WB analysis of human placenta using 11185-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11185-1-AP (SGK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using SGK2 Polyclonal antibody (11185-1-AP)

    WB analysis of mouse pancreas using 11185-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11185-1-AP (SGK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SGK2 Polyclonal antibody (11185-1-AP)

    IHC staining of human pancreas cancer using 11185-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11185-1-AP (SGK2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SGK2 Polyclonal antibody (11185-1-AP)

    IHC staining of human pancreas cancer using 11185-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11185-1-AP (SGK2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inhuman placenta tissue, mouse pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11185-1-AP targets SGK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGK2 fusion protein Ag1669 相同性解析による交差性が予測される生物種
Full Name serum/glucocorticoid regulated kinase 2
Calculated molecular weight 48 kDa
GenBank accession numberBC014037
Gene symbol SGK2
Gene ID (NCBI) 10110
RRIDAB_2188525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SGK2 antibody 11185-1-APDownload protocol
IHC protocol for SGK2 antibody 11185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Autophagy

Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits.

Authors - Seonghee Jung
  • KO Validated
View Article