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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SGLT1 Polyclonal antibody

SGLT1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 30861-1-AP

Synonyms

SLC5A1, High affinity sodium-glucose cotransporter

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SGLT1 Polyclonal antibody (30861-1-AP)

    WB analysis using 30861-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SGLT1 Polyclonal antibody (30861-1-AP)

    WB analysis using 30861-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using mouse SGLT1 Polyclonal antibody (30861-1-AP)

    IP experiment of mouse kidney using 30861-1-AP

    IP result of anti-SGLT1 (IP:30861-1-AP, 4ug; Detection:30861-1-AP 1:400) with mouse kidney tissue lysate 2160 ug.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SGLT1 Polyclonal antibody (30861-1-AP)

    IHC staining of mouse small intestine using 30861-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SGLT1 Polyclonal antibody (30861-1-AP)

    IHC staining of mouse small intestine using 30861-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse colon tissue, mouse kidney tissue, mouse small intestine tissue, rat colon tissue
Positive IP detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

30861-1-AP targets SGLT1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitypig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGLT1 fusion protein Ag29338 相同性解析による交差性が予測される生物種
Full Name solute carrier family 5 (sodium/glucose cotransporter), member 1
Calculated molecular weight 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberNM_000343
Gene symbol SGLT1
Gene ID (NCBI) 6523
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC5A1, also known as SGLT1, is a sodium-dependent glucose transporter (SGLT) family member. SGLT1 couples sugar transport to Na+ gradients across the intestinal brush border (PMID: 8563765). SGLT1 mediates active glucose and galactose absorption in the intestine and renal glucose scavenging. Mutations in SGLT1 cause glucose-galactose malabsorption (GGM) syndrome (PMID: 34880492). SGLT1 is expressed in intestine and endometrial cells (PMID: 2490366, 28974690).

Protocols

Product Specific Protocols
WB protocol for SGLT1 antibody 30861-1-APDownload protocol
IP protocol for SGLT1 antibody 30861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

J Anim Sci

The Inflammatory Injury of Porcine Small Intestinal Epithelial Cells Induced by Deoxynivalenol is Related to the Decrease of Glucose Transport

Authors - Xiaopeng Tang
View Article