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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SGLT2 Polyclonal antibody

SGLT2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28683-1-AP

Synonyms

Na(+)/glucose cotransporter 2, SGLT2, SLC5A2, Sodium/glucose cotransporter 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SGLT2 Polyclonal antibody (28683-1-AP)

    WB analysis using 28683-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28683-1-AP (SGLT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SGLT2 Polyclonal antibody (28683-1-AP)

    IHC staining of human kidney using 28683-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28683-1-AP (SGLT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SGLT2 Polyclonal antibody (28683-1-AP)

    IHC staining of mouse kidney using 28683-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28683-1-AP (SGLT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using SGLT2 Polyclonal antibody (28683-1-AP)

    IF Staining of mouse kidney using 28683-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SGLT2 antibody (28683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat kidney tissue using SGLT2 Polyclonal antibody (28683-1-AP)

    IF Staining of rat kidney using 28683-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat kidney tissue using SGLT2 antibody (28683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue, rat kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28683-1-AP targets SGLT2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGLT2 fusion protein Ag30120 相同性解析による交差性が予測される生物種
Full Name solute carrier family 5 (sodium/glucose cotransporter), member 2
Calculated molecular weight 672 aa, 73 kDa
Observed molecular weight73 kDa
GenBank accession numberBC131542
Gene symbol SGLT2
Gene ID (NCBI) 6524
RRIDAB_2918190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sodium-glucose cotransporter 2 (SGLT2), encoded by SLC5A2, utilizes the electrochemical sodium gradient to transport glucose against the cell's internal concentration gradient. Mainly expressed on brush border membrane (BBM) of epithelial cells in the early segment of the proximal tubule, SGLT2 mediates most of the glucose reabsorption by the kidney overall. Inhibition of SGLT2 could improve glucose homeostasis of diabetic patients, which has been considered as a novel strategy for diabetes treatment.

Protocols

Product Specific Protocols
WB protocol for SGLT2 antibody 28683-1-APDownload protocol
IHC protocol for SGLT2 antibody 28683-1-APDownload protocol
IF protocol for SGLT2 antibody 28683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Basic Res Cardiol

Empagliflozin prevents heart failure through inhibition of the NHE1-NO pathway, independent of SGLT2

Authors - Sha Chen
View Article