Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells, human skeletal muscle tissue, mouse liver tissue, SH-SY5Y cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue, human liver cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 15 publications below |
| IHC | See 8 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
11019-2-AP targets SGTA in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SGTA fusion protein Ag1475 相同性解析による交差性が予測される生物種 |
| Full Name | small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 34-37 kDa |
| GenBank accession number | BC008885 |
| Gene Symbol | SGTA |
| Gene ID (NCBI) | 6449 |
| RRID | AB_2188830 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43765 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SGTA antibody 11019-2-AP | Download protocol |
| IHC protocol for SGTA antibody 11019-2-AP | Download protocol |
| IF protocol for SGTA antibody 11019-2-AP | Download protocol |
| IP protocol for SGTA antibody 11019-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes. | ||
PLoS Pathog A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.
| ||
PLoS Pathog A Non-enveloped Virus Hijacks Host Disaggregation Machinery to Translocate across the Endoplasmic Reticulum Membrane. | ||
Int J Cancer Knockdown of the co-chaperone SGTA results in the suppression of androgen and PI3K/AKT signaling and inhibition of prostate cancer cell proliferation. | ||
Int J Mol Sci Isoquercitrin Attenuates Steatohepatitis by Inhibition of the Activated NLRP3 Inflammasome through HSP90 | ||
Sci Rep Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha (SGTA) Ablation Limits Offspring Viability and Growth in Mice.
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