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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

SGTA Monoclonal antibody

SGTA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B10B11

Cat no : 60305-1-Ig

Synonyms

Alpha SGT, alphaSGT, hSGT, SGT, SGT1, SGTA, UBP, Vpu binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SGTA Monoclonal antibody (60305-1-Ig)

    WB analysis using 60305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of NIH/3T3 cells using SGTA Monoclonal antibody (60305-1-Ig)

    WB analysis of NIH/3T3 using 60305-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using SGTA Monoclonal antibody (60305-1-Ig)

    WB analysis of Neuro-2a using 60305-1-Ig

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using SGTA Monoclonal antibody (60305-1-Ig)

    WB analysis of fetal human brain using 60305-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SGTA Monoclonal antibody (60305-1-Ig)

    IHC staining of human liver cancer using 60305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60305-1-Ig (SGTA Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SGTA Monoclonal antibody (60305-1-Ig)

    IHC staining of human liver cancer using 60305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60305-1-Ig (SGTA Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SGTA Monoclonal antibody (60305-1-Ig)

    IF Staining of HeLa using 60305-1-Ig

    Immunofluorescent analysis of HeLa cells using 60305-1-Ig (SGTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L).

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Tested Applications

Positive WB detected inLNCaP cells, fetal human brain tissue, NIH/3T3 cells, Neuro-2a cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60305-1-Ig targets SGTA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SGTA fusion protein Ag1475 相同性解析による交差性が予測される生物種
Full Name small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
Calculated molecular weight 34 kDa
Observed molecular weight 34-37 kDa
GenBank accession numberBC008885
Gene symbol SGTA
Gene ID (NCBI) 6449
RRIDAB_2881419
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.

Protocols

Product Specific Protocols
WB protocol for SGTA antibody 60305-1-IgDownload protocol
IHC protocol for SGTA antibody 60305-1-IgDownload protocol
IF protocol for SGTA antibody 60305-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols