Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human ovary cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
12226-1-AP targets SH2B1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SH2B1 fusion protein Ag2863 相同性解析による交差性が予測される生物種 |
| Full Name | SH2B adaptor protein 1 |
| Calculated molecular weight | 426 aa, 45 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC010704 |
| Gene Symbol | SH2B1 |
| Gene ID (NCBI) | 25970 |
| RRID | AB_2877838 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRF2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SH2B1 is a member of the SH2-domain containing mediators family. SH2B1 has been shown to interact with INS receptor, Grb2, TrkA and Janus kinase 2. Variations close to or in the SH2B1 gene have been found to associate with obesity in two large genome wide association studies of body mass index (PMID: 19079260). SH2B1deletions are associated with severe early-onset obesity (PMID: 19966786).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SH2B1 antibody 12226-1-AP | Download protocol |
| IHC protocol for SH2B1 antibody 12226-1-AP | Download protocol |
| IF protocol for SH2B1 antibody 12226-1-AP | Download protocol |
| IP protocol for SH2B1 antibody 12226-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |