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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SH2D3A Polyclonal antibody (15641-1-AP)

    WB analysis of HeLa using 15641-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SH2D3A Polyclonal antibody (15641-1-AP)

    WB analysis of HepG2 using 15641-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SH2D3A Polyclonal antibody (15641-1-AP)

    WB analysis of HEK-293 using 15641-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SH2D3A Polyclonal antibody (15641-1-AP)

    IHC staining of human ovary cancer using 15641-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15641-1-AP (SH2D3A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SH2D3A Polyclonal antibody (15641-1-AP)

    IF Staining of HeLa using 15641-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH2D3A antibody (15641-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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SH2D3A Polyclonal antibody

SH2D3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15641-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UNQ175/PRO201, SH2 domain-containing protein 3A, NSP1, Novel SH2-containing protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15641-1-AP targets SH2D3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH2D3A fusion protein Ag8132 相同性解析による交差性が予測される生物種
Full Name SH2 domain containing 3A
Calculated molecular weight 576 aa, 63 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC006281
Gene Symbol SH2D3A
Gene ID (NCBI) 10045
RRIDAB_2286019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRG2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SH2 domain containing 3A (SH2D3A) Is is a novel SH2-containing protein 1 (NSP1) with proline-rich domains and therefore probably involved in the signal-transduction of some ligand-activated tyrosine kinase receptors. Three phosphorylation sites of NSP1 have been found to date. NSP1 may be located at cell membrane play a role in JNK activation.

Protocols

Product Specific Protocols
WB protocol for SH2D3A antibody 15641-1-APDownload protocol
IHC protocol for SH2D3A antibody 15641-1-APDownload protocol
IF protocol for SH2D3A antibody 15641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols