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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SH2D3C Polyclonal antibody

SH2D3C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12882-1-AP

Synonyms

CHAT, Novel SH2 containing protein 3, NSP3, PRO34088, SH2 domain containing 3C, SH2D3C

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SH2D3C Polyclonal antibody (12882-1-AP)

    WB analysis of HEK-293 using 12882-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12882-1-AP (SH2D3C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using SH2D3C Polyclonal antibody (12882-1-AP)

    WB analysis of human brain using 12882-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12882-1-AP (SH2D3C antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SH2D3C Polyclonal antibody (12882-1-AP)

    WB analysis of human placenta using 12882-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12882-1-AP (SH2D3C antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment using SH2D3C Polyclonal antibody (12882-1-AP)

    IP experiment using 12882-1-AP

    IP result of SH2D3C antibody (12882-1-AP for IP and Detection) with mouse brain tissue lysate.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SH2D3C Polyclonal antibody (12882-1-AP)

    IP experiment of mouse brain using 12882-1-AP

    IP result of anti-SH2D3C (IP:12882-1-AP, 3ug; Detection:12882-1-AP 1:500) with mouse brain tissue lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SH2D3C Polyclonal antibody (12882-1-AP)

    IHC staining of human breast cancer using 12882-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12882-1-AP (SH2D3C antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, human placenta tissue
Positive IP detected inmouse brain tissue,
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12882-1-AP targets SH2D3C in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH2D3C fusion protein Ag3887 相同性解析による交差性が予測される生物種
Full Name SH2 domain containing 3C
Calculated molecular weight 94 kDa, 86 kDa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC027962
Gene symbol SH2D3C
Gene ID (NCBI) 10044
RRIDAB_2188993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SH2 domain-containing protein 3C (SH2D3C, or NSP3) gene encodes an adaptor protein and member of a cytoplasmic protein family involved in cell migration. The encoded protein was identified as an Eph receptor-binding protein which may be a positive regulator of TCR signaling (PMID: 10542222). SH2D3C is an adaptor protein that has been implicated in T cell activation and shown to associate with different components of the integrin signaling pathway ex vivo, moreover, its primary in vivo role of Sh2d3c in regulating B cell development and function has also been demonstrated (PMID: 20505138). SH2D3C has 6 isoforms with MW 94, 86, 77 and 55 kDa (refer to UniProt).

Protocols

Product Specific Protocols
WB protocol for SH2D3C antibody 12882-1-APDownload protocol
IHC protocol for SH2D3C antibody 12882-1-APDownload protocol
IP protocol for SH2D3C antibody 12882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IP

Neuroscience

Up-regulation of NSP3 by oligomeric Aβ accelerates neuronal death through Cas-independent Rap1A activation.

Authors - Fujiya Gomi
View Article