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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SH3BP1 Monoclonal antibody, PBS Only

SH3BP1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B12C6

Cat no : 68328-1-PBS

Synonyms

SH3 domain-binding protein 1, SH3 domain binding protein 1, 3BP 1, 1B12C6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SH3BP1 Monoclonal antibody (68328-1-Ig)

    WB analysis using 68328-1-Ig (same clone as 68328-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68328-1-Ig (SH3BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68328-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using SH3BP1 Monoclonal antibody (68328-1-Ig)

    WB analysis of LNCaP using 68328-1-Ig (same clone as 68328-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68328-1-Ig (SH3BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68328-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SH3BP1 Monoclonal antibody (68328-1-Ig)

    WB analysis of HepG2 using 68328-1-Ig (same clone as 68328-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68328-1-Ig (SH3BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68328-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SH3BP1 Monoclonal antibody (68328-1-Ig)

    IF Staining of HeLa using 68328-1-Ig (same clone as 68328-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH3BP1 antibody (68328-1-Ig, Clone: 1B12C6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68328-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using SH3BP1 Monoclonal antibody (68328-1-Ig)

    FC experiment of HepG2 using 68328-1-Ig (same clone as 68328-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SH3BP1 Monoclonal antibody (68328-1-Ig, Clone:1B12C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68328-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68328-1-PBS targets SH3BP1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SH3BP1 fusion protein Ag15165 相同性解析による交差性が予測される生物種
Full Name SH3-domain binding protein 1
Calculated molecular weight 701 aa, 76 kDa
Observed molecular weight76-80 kDa
GenBank accession numberBC008282
Gene symbol SH3BP1
Gene ID (NCBI) 23616
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SH3BP1 is also named as Chimeric SH3BP1-PDXP protein and BARGIN, and belongs to the to the RhoGAP family. SH3BP1 can be as a partner of the exocyst complex and establish a physical and functional link between two motility-driving pathways, the Ral/exocyst and Rac signaling pathways. (PMID:21658605). SH3BP1 localizes together with the exocyst to the leading edge of motile cells and that SH3BP1 regulates cell migration via its GAP activity upon Rac1.