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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SH3BP5 Monoclonal antibody, PBS Only

SH3BP5 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rabbit, mouse, rat, pig, chicken

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B9D4

Cat no : 67841-1-PBS

Synonyms

SAB, SH3 domain binding protein 5, SH3BP 5, SH3BP5

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SH3BP5 Monoclonal antibody (67841-1-Ig)

    WB analysis of mouse brain using 67841-1-Ig (same clone as 67841-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67841-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of chicken brain tissue using SH3BP5 Monoclonal antibody (67841-1-Ig)

    WB analysis of chicken brain using 67841-1-Ig (same clone as 67841-1-PBS)

    chicken brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67841-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SH3BP5 Monoclonal antibody (67841-1-Ig)

    WB analysis using 67841-1-Ig (same clone as 67841-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67841-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit brain tissue using SH3BP5 Monoclonal antibody (67841-1-Ig)

    WB analysis of rabbit brain using 67841-1-Ig (same clone as 67841-1-PBS)

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67841-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67841-1-PBS targets SH3BP5 in WB, Indirect ELISA applications and shows reactivity with Human, rabbit, mouse, rat, pig, chicken samples.

Tested Reactivity Human, rabbit, mouse, rat, pig, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SH3BP5 fusion protein Ag30252 相同性解析による交差性が予測される生物種
Full Name SH3-domain binding protein 5 (BTK-associated)
Calculated molecular weight 47 kDa
Observed molecular weight75 kDa
GenBank accession numberBC010123
Gene symbol SH3BP5
Gene ID (NCBI) 9467
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SH3BP5(SH3 domain-binding protein 5) is also named as SAB. It is previously isolated in a screen for proteins capable of binding to the Src homology 3 (SH3) domain of Bruton's tyrosine kinase (Btk) and could play a role in targeting JNK and Btk to mitochondria and/or mediating cross-talk between the JNK and Btk signalling pathways. It has 2 isoforms produced by alternative splicing with the molecular mass of 50 kDa and 32 kDa. There are two bands of 83 kDa and 70 kDa can be detected in A431 and the larger species may correspond to splice variants of SH3BP5 or SH3BP5 proteins that have undergone post-translational modification(PMID:12167088). In addition, we detect the band of 32 kDa as the isoform 2 of SH3BP5 and another band of 62 kDa besides the 47 kDa and 80 kDa.