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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SH3D19 Polyclonal antibody

SH3D19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21499-1-AP

Synonyms

EVE1, EEN-binding protein, EEN binding protein, EBP, ADAM-binding protein Eve-1

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using SH3D19 Polyclonal antibody (21499-1-AP)

    WB analysis of HT-1080 using 21499-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 21499-1-AP (SH3D19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using SH3D19 Polyclonal antibody (21499-1-AP)

    WB analysis of Y79 using 21499-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 21499-1-AP (SH3D19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using SH3D19 Polyclonal antibody (21499-1-AP)

    IHC staining of human heart using 21499-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using SH3D19 Polyclonal antibody (21499-1-AP)

    IHC staining of human heart using 21499-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SH3D19 Polyclonal antibody (21499-1-AP)

    IHC staining of human kidney using 21499-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SH3D19 Polyclonal antibody (21499-1-AP)

    IHC staining of human kidney using 21499-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SH3D19 Polyclonal antibody (21499-1-AP)

    IF Staining of HepG2 using 21499-1-AP

    Immunofluorescent analysis of HepG2 cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SH3D19 Polyclonal antibody (21499-1-AP)

    IF Staining of HeLa using 21499-1-AP

    Immunofluorescent analysis of Hela cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inHT-1080 cells, Y79 cells
Positive IHC detected inhuman heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21499-1-AP targets SH3D19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH3D19 fusion protein Ag15915 相同性解析による交差性が予測される生物種
Full Name SH3 domain containing 19
Calculated molecular weight 790 aa, 87 kDa
Observed molecular weight 45-47 kDa
GenBank accession numberBC108890
Gene symbol SH3D19
Gene ID (NCBI) 152503
RRIDAB_10733218
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SH3D19 antibody 21499-1-APDownload protocol
IHC protocol for SH3D19 antibody 21499-1-APDownload protocol
IF protocol for SH3D19 antibody 21499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols