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Endophilin 1 Polyclonal antibody
Endophilin 1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12435-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | C6 cells, mouse brain, rat brain |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 1 publications below |
Product Information
12435-1-AP targets Endophilin 1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Endophilin 1 fusion protein Ag3114 相同性解析による交差性が予測される生物種 |
Full Name | SH3-domain GRB2-like 2 |
Calculated molecular weight | 39.8 kDa |
Observed molecular weight | 40 kDa |
GenBank accession number | BC032825 |
Gene symbol | Endophilin 1 |
Gene ID (NCBI) | 6456 |
RRID | AB_2187398 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for Endophilin 1 antibody 12435-1-AP | Download protocol |
IHC protocol for Endophilin 1 antibody 12435-1-AP | Download protocol |
IP protocol for Endophilin 1 antibody 12435-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Neoplasia Loss of Sh3gl2/Endophilin A1 Is a Common Event in Urothelial Carcinoma that Promotes Malignant Behavior.
| ||
Invest Ophthalmol Vis Sci Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma |