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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using SH3GL3 Polyclonal antibody (13849-1-AP)

    WB analysis of human brain using 13849-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13849-1-AP (SH3GL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SH3GL3 Polyclonal antibody (13849-1-AP)

    WB analysis of mouse brain using 13849-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13849-1-AP (SH3GL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using SH3GL3 Polyclonal antibody (13849-1-AP)

    WB analysis of mouse testis using 13849-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13849-1-AP (SH3GL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using SH3GL3 Polyclonal antibody (13849-1-AP)

    WB analysis of human brain using 13849-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13849-1-AP (SH3GL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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SH3GL3 Polyclonal antibody

SH3GL3 Polyclonal Antibody for WB, ELISA
Cat No. 13849-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CNSA3, EEN 2B L3, EEN B2, Endophilin 3, Endophilin A3, HsT19371, SH3 domain GRB2 like 3, SH3 domain protein 2C, SH3D2C, SH3GL3, SH3P13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13849-1-AP targets SH3GL3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH3GL3 fusion protein Ag4819 相同性解析による交差性が予測される生物種
Full Name SH3-domain GRB2-like 3
Calculated molecular weight 39 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC042864
Gene Symbol SH3GL3
Gene ID (NCBI) 6457
RRIDAB_2187423
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99963
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SH3GL3 is a novel member of SH3-containing family that has a src-homology-3 domain (SH3) at the C-terminus and drives protein-protein interactions through binding to proline-rich ligands. SH3GL3 is selectively expressed in brain and testis, and plays important role in signal transduction and regulation of synaptic vesicles.

Protocols

Product Specific Protocols
WB protocol for SH3GL3 antibody 13849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols