Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of mouse kidney using 15422-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 15422-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 15422-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 15422-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 15422-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 15422-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 15422-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 15422-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15422-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15422-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 15422-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 15422-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 15422-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse kidney using 15422-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15422-1-AP (Bif-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 15422-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15422-1-AP (Bif-1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 15422-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15422-1-AP (Bif-1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of rat kidney using 15422-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15422-1-AP (Bif-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15422-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Bif-1 antibody (15422-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Bif-1 Polyclonal antibody

Bif-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15422-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Synonyms

SH3GLB1, SH3 domain-containing GRB2-like protein B1, Endophilin-B1, Endophilin B1, Bif1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, HEK-293 cells, human placenta tissue, mouse heart tissue, mouse placenta tissue
Positive IHC detected in	mouse kidney tissue, human kidney tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 2 publications below
IF	See 2 publications below
ChIP	See 1 publications below

Product Information

15422-1-AP targets Bif-1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Bif-1 fusion protein Ag7710 相同性解析による交差性が予測される生物種
Full Name	SH3-domain GRB2-like endophilin B1
Calculated molecular weight	41 kDa
Observed molecular weight	41 kDa
GenBank accession number	BC007455
Gene Symbol	Bif-1
Gene ID (NCBI)	51100
RRID	AB_2254564
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y371
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SH3GLB1 also known as Bif-1, contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain, but shares no significant homology with members of the Bcl-2 family. SH3GLB1 is involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588).

Protocols

Product Specific Protocols
WB protocol for Bif-1 antibody 15422-1-AP	Download protocol
IHC protocol for Bif-1 antibody 15422-1-AP	Download protocol
IF protocol for Bif-1 antibody 15422-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Biomed Mater Nano implant surface triggers autophagy through membrane curvature distortion to regulate the osteogenic differentiation Authors - Guangwen Li View Article
rat	WB,IHC	Eur J Pharmacol SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation. Authors - Anbo Gao View Article
human	WB	J Exp Clin Cancer Res SH3GLB1-related autophagy mediates mitochondrial metabolism to acquire resistance against temozolomide in glioblastoma. Authors - Chia-Hung Chien View Article
mouse	WB	Oncotarget Autophagy-related gene expression is an independent prognostic indicator of glioma. Authors - Huixue Zhang View Article
human	WB,ChIP	BMC Cancer Role of SH3GLB1 in the regulation of CD133 expression in GBM cells Authors - Chia-Hung Chien View Article
rat	WB,IHC,IF	Adv Sci (Weinh) Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption Authors - Fan Ouyang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse kidney using 15422-1-AP

WB analysis of human placenta using 15422-1-AP

WB analysis of mouse heart using 15422-1-AP

WB analysis of mouse heart using 15422-1-AP

WB analysis of mouse placenta using 15422-1-AP

WB analysis of mouse placenta using 15422-1-AP

WB analysis of mouse kidney using 15422-1-AP

WB analysis of mouse kidney using 15422-1-AP

WB analysis of HEK-293 using 15422-1-AP

WB analysis of HEK-293 using 15422-1-AP

WB analysis of human placenta using 15422-1-AP

WB analysis of mouse heart using 15422-1-AP

WB analysis of mouse heart using 15422-1-AP

IHC staining of mouse kidney using 15422-1-AP

IHC staining of human kidney using 15422-1-AP

IHC staining of human kidney using 15422-1-AP

IHC staining of rat kidney using 15422-1-AP

IF Staining of HepG2 using 15422-1-AP

Bif-1 Polyclonal antibody

Bif-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Nano implant surface triggers autophagy through membrane curvature distortion to regulate the osteogenic differentiation

SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation.

SH3GLB1-related autophagy mediates mitochondrial metabolism to acquire resistance against temozolomide in glioblastoma.

Autophagy-related gene expression is an independent prognostic indicator of glioma.

Role of SH3GLB1 in the regulation of CD133 expression in GBM cells

Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption