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Bif-1 Polyclonal antibody

Bif-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15422-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ChIP, ELISA

SH3GLB1, SH3 domain-containing GRB2-like protein B1, Endophilin-B1, Endophilin B1, Bif1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, human placenta tissue, mouse heart tissue, mouse placenta tissue
Positive IHC detected inmouse kidney tissue, human kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15422-1-AP targets Bif-1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bif-1 fusion protein Ag7710 相同性解析による交差性が予測される生物種
Full Name SH3-domain GRB2-like endophilin B1
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC007455
Gene Symbol Bif-1
Gene ID (NCBI) 51100
RRIDAB_2254564
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y371
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SH3GLB1 also known as Bif-1, contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain, but shares no significant homology with members of the Bcl-2 family. SH3GLB1 is involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588).

Protocols

Product Specific Protocols
WB protocol for Bif-1 antibody 15422-1-APDownload protocol
IHC protocol for Bif-1 antibody 15422-1-APDownload protocol
IF protocol for Bif-1 antibody 15422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biomed Mater

Nano implant surface triggers autophagy through membrane curvature distortion to regulate the osteogenic differentiation

Authors - Guangwen Li
ratWB,IHC

Eur J Pharmacol

SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation.

Authors - Anbo Gao
humanWB

J Exp Clin Cancer Res

SH3GLB1-related autophagy mediates mitochondrial metabolism to acquire resistance against temozolomide in glioblastoma.

Authors - Chia-Hung Chien
mouseWB

Oncotarget

Autophagy-related gene expression is an independent prognostic indicator of glioma.

Authors - Huixue Zhang
humanWB,ChIP

BMC Cancer

Role of SH3GLB1 in the regulation of CD133 expression in GBM cells

Authors - Chia-Hung Chien
ratWB,IHC,IF

Adv Sci (Weinh)

Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption

Authors - Fan Ouyang
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