Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 15897-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15897-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 15897-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15897-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 15897-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15897-1-AP
IP result of anti-SH3GLB2 (IP:15897-1-AP, 4ug; Detection:15897-1-AP 1:1000) with HeLa cells lysate 2600ug.
IHC staining of human placenta using 15897-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15897-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15897-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15897-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH3GLB2 antibody (15897-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

SH3GLB2 Polyclonal antibody

SH3GLB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15897-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PP9455, PP6569, PP578, KIAA1848, Endophilin-B2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human testis tissue, mouse lung tissue, mouse testis tissue, rat lung tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse brain tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IF	See 1 publications below

Product Information

15897-1-AP targets SH3GLB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SH3GLB2 fusion protein Ag8712 相同性解析による交差性が予測される生物種
Full Name	SH3-domain GRB2-like endophilin B2
Calculated molecular weight	395 aa, 44 kDa
Observed molecular weight	47-50 kDa
GenBank accession number	BC014635
Gene Symbol	SH3GLB2
Gene ID (NCBI)	56904
RRID	AB_2187269
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NR46
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).

Protocols

Product Specific Protocols
WB protocol for SH3GLB2 antibody 15897-1-AP	Download protocol
IHC protocol for SH3GLB2 antibody 15897-1-AP	Download protocol
IF protocol for SH3GLB2 antibody 15897-1-AP	Download protocol
IP protocol for SH3GLB2 antibody 15897-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Brain Pathol Neuronal Susceptibility to Beta-Amyloid Toxicity and Ischemic Injury Involves Histone Deacetylase-2 Regulation of Endophilin-B1. Authors - David B Wang View Article
mouse	WB,IF	J Biol Chem Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection. Authors - Jacob M Serfass KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 15897-1-AP

WB analysis of mouse testis using 15897-1-AP

WB analysis of mouse lung using 15897-1-AP

WB analysis of human testis using 15897-1-AP

WB analysis of rat lung using 15897-1-AP

IP experiment of HeLa using 15897-1-AP

IHC staining of human placenta using 15897-1-AP

IHC staining of mouse brain using 15897-1-AP